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PMID: 724510 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A computer assisted method for the determination of restriction enzyme recognifion sites.

Nucleic acids research ·Vol. 5 ·No. 11 ·1978-11-00 ·Pages 4105-27

Gingeras TR, MIlazzo JP, Roberts RJ

Abstract

A computer program has been developed which aids in the determination of restriction enzyme recognition sequences. This is achieved by cleaving DNAs of known sequence with a restriction endonuclease and comparing the fragmentation pattern with a computer-generated set of patterns. The feasibility of this approach has been tested using fragmentation patterns of 0X174 DNA produced by enzymes of both known and unknown specificity. Recognition sequences are predicted for two restriction endonucleases (BbvI and SfaNI) using this method. In addition, recognition sequences are predicted for two other new enzymes (PvuI and MstI) using another computer-assisted method.

MeSH Terms
Base Sequence Computers DNA DNA Restriction Enzymes Methods Oligodeoxyribonucleotides/analysis
Chemicals
Oligodeoxyribonucleotides DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gingeras T R
MIlazzo J P
Roberts R J
References (17)
17 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.11.4105
Published
1978-11-00
Pages
4105-27
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342737
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.11.4105 PMC Full Text View journal details
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