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PMID: 7251142 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular characterization of environmental and nontoxigenic strains of Vibrio cholerae.

Infection and immunity ·Vol. 32 ·No. 2 ·1981-05-00 ·Pages 661-7

Kaper JB, Moseley SL, Falkow S

Abstract

Environmental and nontoxigenic strains of Vibrio cholerae 0-1 were examined for genes homologous to genes encoding Escherichia coli heat-labile enterotoxin (LT). Restriction fragments encoding LT A and B subunits were isolated from the recombinant plasmid EWD299 and labeled in vitro with 32P. These probes were then hybridized to deoxyribonucleic acid extracted from strains of V. cholerae and visualized by autoradiography. None of the nontoxigenic strains of V. cholerae 0-1 from Louisiana, Alabama, Maryland, Guam, Brazil, Bangladesh, or Great Britain hybridized with the LT probes, whereas all toxigenic strains exhibited homology. In addition, strains of V. cholerae non-0-1, "group F" vibrios, V. vulnificus, and Aeromonas hydrophila were tested, and all were negative except two strains of V. cholerae non-0-1. The presence of plasmids did not correlate with toxigenicity or nontoxigenicity in any of the species examined. Thus, it appears that these strains are not simple nontoxigenic mutants, but rather do not possess any genetic material encoding cholera toxin. Such strains therefore cannot revert and serve as a reservoir of cholera.

MeSH Terms
Cholera Toxin/biosynthesis,genetics DNA, Recombinant Genes Nucleic Acid Hybridization Plasmids Vibrio cholerae/classification,genetics,metabolism
Chemicals
DNA, Recombinant Cholera Toxin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kaper J B
Moseley S L
Falkow S
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30 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.32.2.661-667.1981
Published
1981-05-00
Pages
661-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC351498
Subset
IM
Grants
NIAID NIH HHS · AI 07149-02 · United States
External Links
PubMed source DOI: 10.1128/iai.32.2.661-667.1981 PMC Full Text View journal details
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