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PMID: 72776 Published · ppublish English Journal Article

Idiotypes of inulin-binding myeloma proteins localized to variable region light and heavy chains: genetic significance.

The Journal of experimental medicine ·Vol. 146 ·No. 5 ·1977-11-01 ·Pages 1294-304

Lieberman R, Vrana M, Humphrey W, Chien CC, Potter M

Abstract

Idiotypes of inulin-binding myeloma proteins (InuBMP) were determined primarly by variable region light chains (VL) or by variable region heavy chains (VH) but needed both chains to be expressed. Recombinant molecules were used to show that individual idiotypes (IdI) of U61, E109, T957, and A4 InuBMP and cross-specific idiotypes (IdXB) of U61 were primarily determined by VL while cross-specific idiotype (IdXA) of A4 was determined mainly by VH. The assignment of genes controlling idiotypes to VH based on allotype linkage (e.g., IdXB) is dubious until the role of the L chain in determining that idiotype is assessed. IdXB has been shown to be a VL-VH marker which presumably is controlled by two unlinked genes. However IdXB can be used as a L chain marker in combinations of strains differing in their L chain genes but having the same permissive H chain genes. Conversely IdXB can be used as a H chain marker in strains having the same permissive L chain genes but differing in their H chain genes.

MeSH Terms
Animals Binding Sites, Antibody Cross Reactions Epitopes Genes Immunoglobulin Heavy Chains Immunoglobulin Light Chains Immunoglobulin Variable Region Inulin/metabolism Mice Mice, Inbred BALB C Myeloma Proteins/immunology,metabolism Recombination, Genetic
Chemicals
Epitopes Immunoglobulin Heavy Chains Immunoglobulin Light Chains Immunoglobulin Variable Region Myeloma Proteins Inulin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lieberman R
Vrana M
Humphrey W
Chien C C
Potter M
References (38)
38 references, click to expand
  1. Inheritance of antibody specificity. II. Anti-(4-hydroxy-5-bromo-3-nitrophenyl) acetyl in the mouse.
    J Exp Med. 1975 Apr 1;141(4):840-54 PMID: 47886
  2. Cross idiotypic specificity among heavy chains of macroglobulins with blood group I and i specificities.
    Nature. 1975 Jun 12;255(5509):562-4 PMID: 49852
  3. Antibody activity of heavy and light chains and recombined IgG of human IgG anti-D.
    Scand J Immunol. 1976;5(1-2):155-60 PMID: 57638
  4. Antigen binding and idiotypic properties of reconstituted immunoglobulins G derived from homogeneous rabbit anti-pneumococcal antibodies.
    Eur J Immunol. 1975 Mar;5(3):206-10 PMID: 61867
  5. Subunit interactions in mouse myeloma proteins with anti-galactan activity.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):932-6 PMID: 815909
  6. Idiotype of inulin-binding antibodies and myeloma proteins controlled by genes linked to the allotype locus of the mouse.
    J Immunol. 1976 Dec;117(6):2105-11 PMID: 993595
  7. Multiple individual and cross-specific indiotypes on 13 levan-binding myeloma proteins of BALB/c mice.
    J Exp Med. 1975 Jul 1;142(1):106-19 PMID: 1151286
  8. Purification of homogeneous murine immunoglobulins with anti-fructofuranan specificity.
    J Immunol. 1976 Jun;116(6):1662-3 PMID: 1270808
  9. Quantitative investigations of idiotypic antibodies. IV. Inhibition by specific haptens of the reaction of anti-hapten antibody with its anti-idiotypic antibody.
    J Exp Med. 1970 Nov;132(5):951-62 PMID: 4097134
  10. Common individual antigenic determinants in five of eight BALB-c IgA myeloma proteins that bind phosphoryl choline.
    J Exp Med. 1970 Oct 1;132(4):737-51 PMID: 4101363
  11. Recovery of binding activity in reconstituted mouse myeloma proteins.
    Biochemistry. 1971 Jun 22;10(13):2525-30 PMID: 4104270
  12. The inheritance of individual antigenic specificities of rabbit antibodies to streptococcal carbohydrates.
    J Exp Med. 1971 Aug 1;134(2):532-52 PMID: 4104426
  13. Quantitative investigations of idiotypic antibodies. VI. Idiotypic specificity as a potential genetic marker for the variable regions of mouse immunoglobulin polypeptide chains.
    J Exp Med. 1972 Mar 1;135(3):579-95 PMID: 4110016
  14. Persistence and characterization of idiotypes in pedigreed rabbits producing antibodies of restricted heterogeneity to pneumococcal polysaccharides.
    J Immunol. 1972 May;108(5):1278-87 PMID: 4112921
  15. Antigen-binding IgA myeloma proteins in mice: specificities to antigens containing -D 1 leads to 6 linked galactose side chains and a protein antigen in wheat.
    J Immunol. 1972 Feb;108(2):295-300 PMID: 4114953
  16. Inheritance of an idiotype associated with the immune response of inbred mice to phosphorylcholine.
    Eur J Immunol. 1972 Aug;2(4):319-26 PMID: 4117282
  17. Immunochemical analysis of the cross-reacting idiotypes of mouse myeloma proteins with anti-dextran activity and normal anti-dextran antibody.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):235-9 PMID: 4119224
  18. Evidence for the linkage of the IGC H locus to a gene controlling the idiotypic specificity of anti-p-azophenylarsonate antibodies in strain A mice.
    J Exp Med. 1973 Jan 1;137(1):22-31 PMID: 4120094
  19. Distribution of a cross-reactive idiotypic specificity in inbred strains of mice.
    J Exp Med. 1973 Apr 1;137(4):855-69 PMID: 4120895
  20. Localization of idiotypic antigenic determinants in the Fv region of murine myeloma protein MOPC-315.
    Proc Natl Acad Sci U S A. 1973 May;70(5):1585-7 PMID: 4123072
  21. Genetics of a new IgVH (T15 idiotype) marker in the mouse regulating natural antibody to phosphorylcholine.
    J Exp Med. 1974 Apr 1;139(4):983-1001 PMID: 4131516
  22. Inheritance of antibody specificity. I. Anti-(4-hydroxy-3-nitrophenyl)acetyl of the mouse primary response.
    J Exp Med. 1974 Dec 1;140(6):1498-510 PMID: 4139230
  23. Structure of antibodies with shared idiotypy: the complete sequence of the heavy chain variable regions of two immunoglobulin M anti-gamma globulins.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4032-6 PMID: 4139708
  24. Specificity of recombination of H and L chains from human gamma-G-myeloma proteins.
    J Exp Med. 1965 Sep 1;122(3):619-32 PMID: 4158438
  25. Preferential recombination of antibody chains to form effective binding sites.
    J Exp Med. 1965 Oct 1;122(4):785-98 PMID: 4158753
  26. Recovery of antibody activity from inactive hybrids of H and L chains.
    J Exp Med. 1967 Jan 1;125(1):191-7 PMID: 4163359
  27. Antigenic specificities related to the cold agglutinin activity of gamma M globulins.
    Science. 1968 Jul 26;161(3839):379-81 PMID: 4173573
  28. Chain interactions and idiotypic specificities of homogeneous rabbit anti-lactose antibodies.
    J Immunol. 1974 Jul;113(1):162-72 PMID: 4208917
  29. Variable region sequence of the heavy chain from a phosphorylcholine binding myeloma protein.
    Biochemistry. 1974 Sep 10;13(19):4033-8 PMID: 4213527
  30. Idiotypy of rabbit antibodies. I. Comparison of idiotypy of antibodies against Salmonella typhi with that of antibodies against other bacteria in the same rabbits, or of antibodies against Salmonella typhi in various rabbits.
    J Exp Med. 1969 Sep 1;130(3):595-617 PMID: 4390062
  31. Similarities in the light chains of anti-gamma-globulins showing cross-idiotypic specificities.
    J Exp Med. 1974 Jan 1;139(1):128-36 PMID: 4543460
  32. Spectral changes on binding of oligosaccharides to murine immunoglobulin A myeloma proteins.
    Biochemistry. 1973 Jul 31;12(16):3039-44 PMID: 4581145
  33. Idiotypic cross-reactions among anti-streptococcal antibodies in an inbred rabbit population.
    Scand J Immunol. 1974;3(4):483-90 PMID: 4852349
  34. Experimental approaches tohomogenous antibody populations. iIndividual antigenic specificity, cross specificity and diversity of human antibodies.
    Fed Proc. 1970 Jan-Feb;29(1):55-8 PMID: 4983814
  35. Idiotypic identity of antibodies to streptococcal carbohydrate in inbred mice.
    Eur J Immunol. 1972 Aug;2(4):301-7 PMID: 5083512
  36. Affinity labeling and cross-linking of the heavy and light chains of a myeloma protein with anti-2,4-dinitrophenyl activity.
    Biochemistry. 1971 Aug 31;10(18):3461-6 PMID: 5118628
  37. INDIVIDUAL ANTIGENIC SPECIFICITY OF MYELOMA PROTEINS. CHARACTERISTICS AND LOCALIZATION TO SUBUNITS.
    J Exp Med. 1965 Apr 1;121:561-75 PMID: 14276777
  38. Individual Antigenic Specificity of Isolated Antibodies.
    Science. 1963 Jun 14;140(3572):1218-9 PMID: 17837506
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.146.5.1294
Published
1977-11-01
Pages
1294-304
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2180964
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.146.5.1294 PMC Full Text View journal details
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