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PMID: 7279669 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Oligonucleotide directed mutagenesis of the human beta-globin gene: a general method for producing specific point mutations in cloned DNA.

Nucleic acids research ·Vol. 9 ·No. 15 ·1981-08-11 ·Pages 3647-56

Wallace RB, Schold M, Johnson MJ, Dembek P, Itakura K

Abstract

A nonadecanucleotide has been used both as a site specific mutagen to introduce a T leads to A transversion mutation in the human beta-globin gene cloned in pBR322 as well as a probe to screen transformed colonies for the desired mutant. The specificity of the oligonucleotide as a mutagen and as a hybridization probe provide a general method for producing site specific mutations in DNA cloned in plasmid vectors such as pBR322.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Recombinant/metabolism Genes/drug effects Globins/genetics Humans Mutagens Mutation Nucleic Acid Hybridization Oligonucleotides/pharmacology Oligoribonucleotides/pharmacology Plasmids
Chemicals
DNA, Recombinant Mutagens Oligonucleotides Oligoribonucleotides Globins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wallace R B
Schold M
Johnson M J
Dembek P
Itakura K
References (22)
22 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.15.3647
Published
1981-08-11
Pages
3647-56
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327381
Subset
IM
Grants
NCI NIH HHS · CA16434 · United States
NIGMS NIH HHS · GM26391 · United States
NHLBI NIH HHS · HL24235 · United States
External Links
PubMed source DOI: 10.1093/nar/9.15.3647 PMC Full Text View journal details
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