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PMID: 7287183 Published · ppublish English Journal Article

Polymorphonuclear neutrophil chemotaxis induced and inhibited by Bacteroides spp.

Infection and immunity ·Vol. 33 ·No. 3 ·1981-09-00 ·Pages 806-10

Adamu SA, Sperry JF

Abstract

A possible virulence factor for Bacteroides subcutaneous abscesses has been found. The effect of Bacteroides culture filtrate and outer membrane on chemotaxis of rabbit peritoneal polymorphonuclear (PMN) neutrophils was assayed in Boyden chambers and via exocytosis of N-acetyl-beta-glucosaminidase. Both Bacteroides culture filtrate and outer membrane elicited some chemotaxis, as measured in the Boyden chamber; however, they had little effect upon exocytosis in cytochalasin B-treated PMN neutrophils. In the presence of serum complement, they completely abolished PMN neutrophil movement in the Boyden chamber assay, yet they gave a definite positive response for the exocytosis assay in the presence of serum complement or activated complement fragment.

MeSH Terms
Acetylglucosaminidase/metabolism Animals Bacteroides/physiology Bacteroides fragilis/physiology Cell Membrane/physiology Chemotaxis, Leukocyte Cytochalasin B/pharmacology Exocytosis Lipopolysaccharides/physiology Neutrophils/enzymology,physiology Rabbits
Chemicals
Lipopolysaccharides Cytochalasin B Acetylglucosaminidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Adamu S A
Sperry J F
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22 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.33.3.806-810.1981
Published
1981-09-00
Pages
806-10
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC350783
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.33.3.806-810.1981 PMC Full Text View journal details
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