Home LiteratureArticle Details
PMID: 7297248 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The structure of the cold-stable kinetochore fiber in metaphase PtK1 cells.

Chromosoma ·Vol. 84 ·No. 1 ·1981-00-00 ·Pages 145-58

Rieder CL

Abstract

When metaphase PtK1 cells are cooled to 6-8 degrees C for 4-6 h the free, polar, and astral spindle microtubules (MTs) disassemble while the MTs of each kinetochore fiber cluster together and persist as bundles of cold-stable MTs. These cold-stable kinetochore fibers are similar to untreated kinetochore fibers in both their length (i.e., 5-6 micrometer) and in the number of kinetochore-associated MTs (i.e., 20-45) of which they are comprised. Quantitative information concerning the lengths of MTs within these fibers was obtained by tracking individual MTs between serial transverse sections. Approximately 1/2 of the kinetochore MTs in each fiber were found to run uninterrupted into the polar region of the spindle. It can be inferred from this and other data that a substantial number of MTs run uninterrupted between the kinetochore and the polar region in untreated metaphase PtK1 cells.

MeSH Terms
Animals Cell Line Cold Temperature Kidney Marsupialia Metaphase Microscopy, Electron Microtubules/ultrastructure
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Rieder C L
References (25)
25 references, click to expand
  1. On the mechanism of anaphase spindle elongation in Diatoma vulgare.
    J Cell Biol. 1977 Aug;74(2):377-88 PMID: 885908
  2. Ultrastructure of the mitotic spindle.
    Int Rev Cytol Suppl. 1977;(6):1-58 PMID: 348627
  3. Pressure-induced depolymerization of spindle microtubules. III. Differential stability in HeLa cells.
    J Cell Biol. 1976 May;69(2):443-54 PMID: 1262399
  4. Micromanipulation studies of chromosome movement. II. Birefringent chromosomal fibers and the mechanical attachment of chromosomes to the spindle.
    J Cell Biol. 1979 Aug;82(2):542-54 PMID: 479316
  5. Procedure for embedding in situ selected cells cultured in vitro.
    J Cell Biol. 1967 Oct;35(1):279-83 PMID: 4863049
  6. Studies on the mechanism of mitosis.
    Ann N Y Acad Sci. 1975 Jun 30;253:407-27 PMID: 1056752
  7. Mitosis in Oedogonium: spindle microfilaments and the origin of the kinetochore fiber.
    Eur J Cell Biol. 1980 Oct;22(2):687-98 PMID: 7192627
  8. Mitotic mechanism based on intrinsic microtubule behaviour.
    Nature. 1978 Mar 30;272(5652):450-2 PMID: 634367
  9. Cold-labile and cold-stable microtubules in the mitotic spindle of mammalian cells.
    Ann N Y Acad Sci. 1975 Jun 30;253:428-39 PMID: 1056753
  10. Mitosis.
    Adv Cell Biol. 1971;2:225-97 PMID: 5005502
  11. Heat-induced reversible hexagonal packing of spindle microtubules.
    J Cell Biol. 1977 Sep;74(3):717-25 PMID: 561787
  12. Micromanipulation studies of chromosome movement. I. Chromosome-spindle attachment and the mechanical properties of chromosomal spindle fibers.
    J Cell Biol. 1979 Aug;82(2):528-41 PMID: 479315
  13. Immunofluorescence localization of HeLa cell microtubule-associated proteins on microtubules in vitro and in vivo.
    J Cell Biol. 1980 Dec;87(3 Pt 1):792-801 PMID: 7007396
  14. The ultrastructure of spindle microtubules after freeze-etching and negative staining in situ.
    J Ultrastruct Res. 1975 Jun;51(3):340-7 PMID: 48555
  15. The pericentriolar material in Chinese hamster ovary cells nucleates microtubule formation.
    J Cell Biol. 1977 Jun;73(3):601-15 PMID: 559676
  16. The arrangement of microtubules and the attachment of chromosomes to the spindle during anaphase in tipulid spermatocytes.
    Chromosoma. 1974 Apr 9;45(3):245-60 PMID: 4837972
  17. Thick and thin serial sectioning for the three-dimensional reconstruction of biological ultrastructure.
    Methods Cell Biol. 1981;22:215-49 PMID: 6167835
  18. Ultrastructural analysis of mitotic spindle elongation in mammalian cells in vitro. Direct microtubule counts.
    J Cell Biol. 1971 Aug;50(2):416-31 PMID: 4939523
  19. The attachment of kinetochores to the pro-metaphase spindle in PtK1 cells. Recovery from low temperature treatment.
    Chromosoma. 1981;82(5):693-716 PMID: 7261715
  20. Light and electron microscopy of rat kangaroo cells in mitosis. I. Formation and breakdown of the mitotic apparatus.
    Chromosoma. 1973;40(1):43-82 PMID: 4344486
  21. Cold-stable microtubules from brain.
    Biochemistry. 1980 Apr 29;19(9):1993-2001 PMID: 7378390
  22. Structural studies on mitotic spindles isolated from cultured human cells.
    J Ultrastruct Res. 1979 Jan;66(1):40-52 PMID: 423322
  23. Functional implications of cold-stable microtubules in kinetochore fibers of insect spermatocytes during anaphase.
    J Cell Biol. 1980 Jun;85(3):853-65 PMID: 7391142
  24. Chromosome movement: current models and experiments on living cells.
    Soc Gen Physiol Ser. 1975;30:97-117 PMID: 1103305
  25. Light and electron microscopy of rat kangaroo cells in mitosis. II. Kinetochore structure and function.
    Chromosoma. 1973;41(2):195-220 PMID: 4571311
Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00293368
Published
1981-00-00
Pages
145-58
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00293368 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2