A rapid and simple method for the isolation of pure eosinophilic leukocytes from horse blood.

Experientia ·Vol. 34 ·No. 12 ·1978-12-15 ·Pages 1654-6

Jörg A, Portmann P, Fellay G, Dreyer JL, Meyer J

Abstract

An improved and short method is described for the isolation of intact eosinophilic leukocytes from horse blood with high yield (1--1.5 g/20 l). Viability and purity of the preparations were verified by light and electron microscopy and by the trypan blue exclusion test. Isolated eosinophils were 98--100% pure, intact and viable, and they could be shown to phagocytise immune-complexes.

MeSH Terms

Animals Cell Separation/methods Centrifugation Eosinophils/cytology Horses

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Jörg A

Portmann P

Fellay G

Dreyer J L

Meyer J

References (4)

4 references, click to expand

Isolation and partial characterization of human eosinophil granules. Comparison to neutrophils.
Am J Pathol. 1975 Dec;81(3):575-88 PMID: 1211424
Studies with eosinophil leucocytes isolated from the blood of the horse.
Br J Haematol. 1960 Jul;6:229-41 PMID: 13684035
Arginine-rich cationic proteins of human eosinophil granules: comparison of the constituents of eosinophilic and neutrophilic leukocytes.
Lab Invest. 1977 May;36(5):493-500 PMID: 194110
Partial characterization of the protein components of eosinophil granules isolated from guinea pig exudates.
Arch Biochem Biophys. 1973 Jun;156(2):383-9 PMID: 4718777

Article Info

Journal

Experientia

Abbr.

Experientia

ISSN

0014-4754

DOI

10.1007/BF02034734

Published

1978-12-15

Pages

1654-6

Language

English

Region

Switzerland

NLM ID

0376547

Subset

External Links

PubMed source DOI: 10.1007/BF02034734

Analysis Services

A rapid and simple method for the isolation of pure eosinophilic leukocytes from horse blood.

Contact

WeChat Official Account