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PMID: 7299348 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Somatically generated mouse myeloma variants synthesizing IgA half-molecules.

The Journal of experimental medicine ·Vol. 154 ·No. 5 ·1981-11-01 ·Pages 1554-69

Zack DJ, Morrison SL, Cook WD, Dackowski W, Scharff MD

Abstract

Whereas mouse myelomas that secrete IgA half-molecules have been shown to arise in vivo, their origin has not been definitely established. We show that somatic variants secreting phenotypically similar molecules can arise directly from the normal IgA-secreting myelomas S107 and W3082. In addition to being improperly assembled, the variant proteins have distinct carboxy-terminal deletions and an aberrant heavy-light chain disulfide bond. For at least one of the variants, variable region serology and affinity for hapten are both unaffected by these changes. Southern and Northern blot analyses indicate normal size DNA restriction fragments and mRNA, suggesting premature termination as the mechanism of deletion. These results are discussed in relation to possible mutational hot spots and long-range interdomain interactions.

MeSH Terms
Animals Carboxypeptidases/metabolism DNA/analysis Genetic Variation Immunoglobulin A/biosynthesis,genetics Immunoglobulin Variable Region/genetics Macromolecular Substances Mice Mice, Inbred BALB C Multiple Myeloma/genetics,immunology RNA, Messenger/analysis
Chemicals
Immunoglobulin A Immunoglobulin Variable Region Macromolecular Substances RNA, Messenger DNA Carboxypeptidases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zack D J
Morrison S L
Cook W D
Dackowski W
Scharff M D
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.154.5.1554
Published
1981-11-01
Pages
1554-69
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2186520
Subset
IM
Grants
NCI NIH HHS · CA 13696 · United States
NCI NIH HHS · CA 16858 · United States
NCI NIH HHS · CA 22736 · United States
External Links
PubMed source DOI: 10.1084/jem.154.5.1554 PMC Full Text View journal details
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