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PMID: 7301595 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Z-DNA conformation of N-2-acetylaminofluorene modified poly(dG-dC).poly(dG-dC) determined by reactivity with anti cytidine antibodies and minimized potential energy calculations.

Nucleic acids research ·Vol. 9 ·No. 20 ·1981-10-24 ·Pages 5459-67

Santella RM, Grunberger D, Broyde S, Hingerty BE

Abstract

The conformation of poly(dG-dC).poly(dG-dC), poly(dG).poly(dC), and calf thymus DNA modified with N-acetoxy-N-2-acetylaminofluorene (N-acetoxy-AAF) was examined by extent of reaction with anti cytidine antibodies. In contrast to modified poly(dG).poly(dC0 and DNA, modified poly(dG-dC).poly (dG-dC) failed to react with the antibodies indicating that the base pairing in this polymer is intact. This in consistent with induction of the Z-DNA conformation in AAF modified poly(dG-dC).poly(dG-dC). Using minimized potential energy calculations on the dCpdG-AAF dimer as a model for the modified polymer, it is shown that the proposed Z-DNA conformation is energetically stable. A model is proposed for an AAF modified tetramer, dGpdCpdGpdC, in which the AAF is external to the Z-DNA duplex.

MeSH Terms
2-Acetylaminofluorene Animals Antibodies Antigen-Antibody Complex Cattle Cytidine/immunology DNA Nucleic Acid Conformation Polydeoxyribonucleotides Radioimmunoassay Thymus Gland
Chemicals
Antibodies Antigen-Antibody Complex Polydeoxyribonucleotides poly(dG).poly(dC) Cytidine DNA 2-Acetylaminofluorene
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Santella R M
Grunberger D
Broyde S
Hingerty B E
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17 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.20.5459
Published
1981-10-24
Pages
5459-67
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327532
Subset
IM
Grants
PHS HHS · 13696 · United States
NCI NIH HHS · 1R01 CA 28038-01A1 · United States
NCI NIH HHS · CA 21111 · United States
External Links
PubMed source DOI: 10.1093/nar/9.20.5459 PMC Full Text View journal details
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