Suppression of the frequencies of sister chromatid exchanges in Bloom's syndrome fibroblasts by co-cultivation with Chinese hamster cells.

Human genetics ·Vol. 44 ·No. 2 ·1978-10-31 ·Pages 187-9

van Buul PP, Natarajan AT, Verdegaal-Immerzeel EA

Abstract

The effect of co-cultivation of Bloom's syndrome fibroblasts with Chinese hamster ovary cells (CHO) on the incidence of sister chromatid exchanges (SCEs) was studied. The results show that suppression of the frequency of SCEs in Bloom's syndrome cells occurs only if cell to cell contact is present with CHO cells, without any effect on the SCE frequency in the latter. It is suggested that possible genetic heterogeneity between different Bloom's syndrome patients can be studied using the method of co-cultivation.

MeSH Terms

Abnormalities, Multiple/genetics Cell Communication Cells, Cultured Crossing Over, Genetic Culture Techniques/methods Dwarfism/genetics Humans Skin Diseases/genetics Syndrome

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

van Buul P P

Natarajan A T

Verdegaal-Immerzeel E A

References (8)

8 references, click to expand

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Transfer of cytoplasmic and nuclear proteins between cells in culture.
J Mol Biol. 1973 Jun 25;78(1):197-210 PMID: 4731020
Progeria cells are stimulated to repair DNA by co-cultivation with normal cells.
Exp Cell Res. 1976 Feb;97(2):291-6 PMID: 1248520
Bloom's syndrome. IV. Sister-chromatid exchanges in lymphocytes.
Am J Hum Genet. 1977 May;29(3):248-55 PMID: 868871
Evidence for transfer of macromolecular RNA between mammalian cells in culture.
Exp Cell Res. 1971 Apr;65(2):313-24 PMID: 4101797
Genetic heterogeneity of xeroderma pigmentosum demonstrated by somatic cell hybridization.
Nat New Biol. 1972 Jul 19;238(81):80-3 PMID: 4505415
A manyfold increase in sister chromatid exchanges in Bloom's syndrome lymphocytes.
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4508-12 PMID: 4140506
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Article Info

Journal

Human genetics

Abbr.

Hum Genet

ISSN

0340-6717

DOI

10.1007/BF00295412

Published

1978-10-31

Pages

187-9

Language

English

Region

Germany

NLM ID

7613873

Subset

External Links

PubMed source DOI: 10.1007/BF00295412 View journal details

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Suppression of the frequencies of sister chromatid exchanges in Bloom's syndrome fibroblasts by co-cultivation with Chinese hamster cells.

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