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PMID: 7305924 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isomyosins in human type 1 and type 2 skeletal muscle fibres.

The Biochemical journal ·Vol. 193 ·No. 1 ·1981-01-01 ·Pages 229-33

Fitzsimons RB, Hoh JF

Abstract

Human myosin from different skeletal muscles was analysed in a non-denaturing gel system, and the isoenzyme composition correlated with the histochemical composition of the muscle. Two components (SM1 and SM2) were associated with type 1 (slow-twitch) fibres, and three (FM1, FM2 and FM3) with type 2 (fast-twitch) fibres. Light-chain analysis was performed in sodium dodecyl sulphate/polyacrylamide gels. There are three light chains (LCs1a, LCS1b and LCs2) in type 1 fibres, and three (LCf1, LCf2 and LCf3) in type 2 fibres. LCf1 and LCs1b co-migrate in sodium dodecyl sulphate gels. The ratio of LCf3/LCf2 is correlated with the distribution of the individual fast isoenzymes. These results explain apparent discrepancies in the literature concerning the light-chain distribution of human myosin.

MeSH Terms
Electrophoresis Electrophoresis, Polyacrylamide Gel Histocytochemistry Humans Isoenzymes Isomerism Muscles/cytology,pathology Myosins
Chemicals
Isoenzymes Myosins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fitzsimons R B
Hoh J F
References (23)
23 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1930229
Published
1981-01-01
Pages
229-33
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1162594
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1930229 PMC Full Text View journal details
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