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PMID: 7317691 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Metabolites of arachidonic acid formed by human gastrointestinal tissues and their actions on the muscle layers.

British journal of pharmacology ·Vol. 74 ·No. 2 ·1981-10-00 ·Pages 435-44

Bennett A, Hensby CN, Sanger GJ, Stamford IF

Abstract

1. Gas chromatography-mass spectrometry demonstrated the presence of arachidonic acid (AA), 6-keto-prostaglandin F1 alpha and thromboxane B2 (TxB2) in all extracts of homogenized muscle or mucosa from human stomach, terminal ileum or sigmoid colon. Prostaglandin D2 (PGD2), PGE2 or PGF2 alpha were usually found more often in the mucosal extracts. The 12-hydroxy-derivative of AA (12-HETE) was detected in all extracts of the colon but in only some of the other tissues. 2. Most prostanoids tested contracted the longitudinal muscle, the order of potency being U-46619 (an epoxymethano analogue of PGH2) greater than PGE2 greater than PGF2 alpha greater than PGD2; PGI2 usually caused relaxation, whereas its breakdown products or TxB2 had weak and variable effects. 3. U-46619 or, less potently, PGF2 alpha contracted the circular muscle, whereas PGI2 and usually PGE2 caused relaxation. PGD2, 6-keto-PGF1 alpha, 6,15-diketo-PGF1 alpha or TxB2 usually had little or no effect. 4. PGI2 antagonized contractions to some excitatory prostanoids, without greatly affecting contractions to acetylcholine. 5. For both muscle layers there was a gradient in sensitivity to prostanoids along the gastrointestinal tract. The sensitivities were stomach greater than distal ileum greater than sigmoid colon. 6. The results are discussed in relation to gastrointestinal physiology and pathophysiology.

MeSH Terms
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid Adult Aged Arachidonic Acids/metabolism,pharmacology Digestive System/drug effects,metabolism Female Gas Chromatography-Mass Spectrometry Humans Leukotrienes Male Middle Aged Muscle Contraction/drug effects Muscle, Smooth/drug effects Peroxides/pharmacology Prostaglandin Endoperoxides, Synthetic/pharmacology Prostaglandins/pharmacology
Chemicals
Arachidonic Acids Leukotrienes Peroxides Prostaglandin Endoperoxides, Synthetic Prostaglandins 12-HPETE 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bennett A
Hensby C N
Sanger G J
Stamford I F
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43 references, click to expand
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Article Info
Journal
British journal of pharmacology
Abbr.
Br J Pharmacol
ISSN
0007-1188
DOI
10.1111/j.1476-5381.1981.tb09989.x
Published
1981-10-00
Pages
435-44
Language
English
Region
England
NLM ID
7502536
PMCID
PMC2071735
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1476-5381.1981.tb09989.x PMC Full Text View journal details
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