The selective release of phospholipase A2 by resident mouse peritoneal macrophages.

The Biochemical journal ·Vol. 200 ·No. 2 ·1981-11-15 ·Pages 441-4

Wightman PD, Dahlgren ME, Davies P, Bonney RJ

Abstract

Resident mouse peritoneal macrophages have three phospholipase activities: a phospholipase A2 active at pH 4.5, a Ca2+-dependent phospholipase A2 active at pH 8.5 and a phosphatidylinositol-specific phospholipase C activity. When macrophages are exposed to zymosan in culture, the cellular activity of pH-4.5 phospholipase A2 is diminished in a manner dependent on zymosan concentration and time of exposure, whereas the cellular activities of pH-8.5 phospholipase A2 and phospholipase C remain unchanged. The depletion of pH-4.5 phospholipase A2 activity from the cell is paralleled by a quantitative recovery of this activity in the culture medium in a manner similar to the cellular depletion and extracellular recovery of two lysosomal enzymes. This release is specifically elicited by an inflammatory substance such as zymosan, since macrophages incubated with 6 micrometer latex spheres retain pH-4.5 phospholipase A2 activity and lysosomal enzyme activities intracellularly.

MeSH Terms

Animals Ascitic Fluid/cytology,enzymology Cells, Cultured Culture Media/metabolism Guinea Pigs Hydrogen-Ion Concentration Macrophages/drug effects,enzymology Male Mice Mice, Inbred Strains Phospholipases/metabolism Phospholipases A/metabolism Phospholipases A2 Zymosan/pharmacology

Chemicals

Culture Media Zymosan Phospholipases Phospholipases A Phospholipases A2

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wightman P D

Dahlgren M E

Davies P

Bonney R J

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2000441

Published

1981-11-15

Pages

441-4

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1163555

Subset

External Links

PubMed source DOI: 10.1042/bj2000441 PMC Full Text View journal details

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The selective release of phospholipase A2 by resident mouse peritoneal macrophages.

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