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PMID: 7399692 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Natural and immune cytolysis of canine distemper virus-infected target cells.

Infection and immunity ·Vol. 28 ·No. 3 ·1980-06-00 ·Pages 724-34

Shek WR, Schultz RD, Appel MJ

Abstract

Natural and immune cytolysis of canine distemper virus (CDV)-infected target cells in vitro is described. Lymphocytes expressing natural cytotoxicity were found in specific-pathogen-free beagle dogs and in beagle-coonhound crosses before vaccination with CDV and indefinitely after vaccination, when the ephemeral immune lymphocyte-mediated cytotoxicity (ILMC) had declined. In contrast to the natural lymphocyte-mediated cytotoxicity, the ILMC was genetically restricted, could not be blocked by CDV-specific antibody, and was effective against measles virus-infected as well as CDV-infectd target cells. Lymphocyte populations were depleted of Fc receptor and surface immunoglobulin-bearing cells by rosetting techniques and tested in comparison. An antibody-dependent cell-mediated cytotoxicity was demostrated against CDV-infected target cells that were preincubated with CDV antibody when Fc receptor-bearing lymphocytes were not removed. The ILMC was measurable for approximately 10 days beginning at 6 days post-vaccination. In contrast, CDV antibody measured by virus neutralization and humoral cytotoxicity was detectable by 6 days postvaccination and persisted at peak levels for at least 5 months.

MeSH Terms
Animals Antibody-Dependent Cell Cytotoxicity Antigens, Surface Antigens, Viral Cells, Cultured Cytotoxicity, Immunologic Distemper/immunology Distemper Virus, Canine/immunology Dogs Lymphocytes/immunology Measles virus/immunology Receptors, Fc Vaccination
Chemicals
Antigens, Surface Antigens, Viral Receptors, Fc
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shek W R
Schultz R D
Appel M J
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.28.3.724-734.1980
Published
1980-06-00
Pages
724-34
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC551011
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.28.3.724-734.1980 PMC Full Text View journal details
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