Human cutaneous lieshmania in a mouse macrophage line: propagation and isolation of intracellular parasites.

Science (New York, N.Y.) ·Vol. 209 ·No. 4462 ·1980-09-12 ·Pages 1240-42

Chang KP

Abstract

A mouse macrophage line, J774G8, supports continuous and prolific intracellular growth of Leishmania mexicana amazonensis, the etiological agent of a South American cutaneous leishmaniasis. The intracellular parasites from these infected cultures can be isolated with high recovery rate and purity by simple Percoll gradient centrifugation.

MeSH Terms

Animals Cells, Cultured Disease Models, Animal Humans Leishmania/growth & development Leishmaniasis/parasitology,pathology Macrophages/parasitology Mice

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Chang K P

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.7403880

Published

1980-09-12

Pages

1240-42

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.7403880 View journal details

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Human cutaneous lieshmania in a mouse macrophage line: propagation and isolation of intracellular parasites.

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