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PMID: 7410477 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation of mouse myocardial gap junctions.

The Journal of cell biology ·Vol. 86 ·No. 3 ·1980-09-00 ·Pages 755-64

Kensler RW, Goodenough DA

Abstract

A new method is presented for the isolation of an enriched fraction of mouse myocardial gap junctions without the use of exogenous proteases. The junctions appear well preserved morphologically and similar to their appearance in situ. Contaminants of the preparation include fragments of the fascia adherens region of the intercalated disk. SDS polyacrylamide gel electrophoresis of the preparation reveals seven major bands with apparent mol wt of 28,000; 31,000; 33,500; 43,000; 47,000; 49,000; and 57,000. Only the bands at 38,000; 31,000; 33,500; and possibly the diffuse band at 47,000 copurify with the morphologically assayed gap junctions. Evidence is presented that the peptides at 43,000 and 57,000 are contained within the contaminating fascia adherens.

MeSH Terms
Animals Cell Fractionation/methods Electrophoresis, Polyacrylamide Gel Female Intercellular Junctions/ultrastructure Male Membrane Proteins/isolation & purification Mice Microscopy, Electron Molecular Weight Myocardium/ultrastructure
Chemicals
Membrane Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kensler R W
Goodenough D A
References (38)
38 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.86.3.755
Published
1980-09-00
Pages
755-64
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110684
Subset
IM
Grants
NIGMS NIH HHS · GM18974 · United States
NIGMS NIH HHS · RCDA GM00231 · United States
External Links
PubMed source DOI: 10.1083/jcb.86.3.755 PMC Full Text View journal details
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