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PMID: 7410482 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Redistribution of membrane proteins in isolated mouse intestinal epithelial cells.

The Journal of cell biology ·Vol. 86 ·No. 3 ·1980-09-00 ·Pages 849-57

Ziomek CA, Schulman S, Edidin M

Abstract

Single mouse intestinal epithelial cells (IEC) may be isolated by the use of a combination of methods used for the isolation of IEC from other species. Isolated cells remain viable for several hours. The membrane integral enzymes alkaline phosphatase and leucine aminopeptidase of isolated IEC are localized to the brush borders of IEC in tissue and in most newly isolated IEC. With time, both enzymes are found distributed over the entire cell surface. Redistribution appears to occur by diffusion in the plane of the membrane. It is slowed, but not blocked, if cells are maintained at 0 degrees C instead of at 37 degrees C, and it is not blocked by fixation in 0.5-3% paraformaldehyde. Drugs that alter cell membrane potential or that affect cell levels of ATP enhance the rate of redistribution of the enzymes.

MeSH Terms
Alkaline Phosphatase/metabolism Animals Cell Membrane/enzymology Cell Separation Diffusion Epithelium/enzymology Intestinal Mucosa/ultrastructure Kinetics Leucyl Aminopeptidase/metabolism Membrane Fluidity Mice Microscopy, Electron Microvilli/enzymology
Chemicals
Alkaline Phosphatase Leucyl Aminopeptidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ziomek C A
Schulman S
Edidin M
References (33)
33 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.86.3.849
Published
1980-09-00
Pages
849-57
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110677
Subset
IM
Grants
NIAID NIH HHS · AI-14584 · United States
External Links
PubMed source DOI: 10.1083/jcb.86.3.849 PMC Full Text View journal details
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