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PMID: 7433090 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complete nucleotide sequence of alfalfa mosaic virus RNA 4.

Nucleic acids research ·Vol. 8 ·No. 10 ·1980-05-24 ·Pages 2213-23

Brederode FT, Koper-Zwarthoff EC, Bol JF

Abstract

Alfalfa mosaic virus RNA 4, the subgenomic messenger for viral coat protein, was partially digested with RNase T1 or RNase A and the sequence of a number of fragments was deduced by in vitro labeling with polynucleotide kinase and application of RNA sequencing techniques. From overlapping fragments, the complete primary sequence of the 881 nucleotides of RNA 4 was constructed: the coding region of 660 nucleotides (not including the initiation and termination codon) is flanked by a 5' noncoding region of 39 nucleotides and a 3' noncoding region of 182 nucleotides. The RNA sequencing data completely confirm the amino acid sequence of the coat protein as deduced by Van Beynum et al. (Fur.J. Biochem. 72, 63-78, 1977).

MeSH Terms
Base Sequence Genes, Viral Medicago sativa Mosaic Viruses/genetics RNA, Viral/genetics Viral Proteins/genetics
Chemicals
RNA, Viral Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brederode F T
Koper-Zwarthoff E C
Bol J F
References (19)
19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1980-05-24
Pages
2213-23
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324073
Subset
IM
Databases
GENBANK
J02006, L00162
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