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PMID: 7433124 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Methylated bases in mycoplasmal DNA.

Nucleic acids research ·Vol. 8 ·No. 6 ·1980-03-25 ·Pages 1383-90

Razin A, Razin S

Abstract

The DNAs of four Mycoplasma and one Acholeplasma species were found to contain methylated bases. All of the five species contained 6-methyladenine (m6Ade), the methylated base characteristic of prokaryotic DNA. The extent of methylation of adenine residues in the mycoplasmal DNA ranged from 0.2% in Mycoplasma capricolum to about 2% in Mycoplasma arginini and Mycoplasma hyorhinis with intermediate methylation values for Mycoplasma orale and Acholeplasma laidlawii DNAs. About 5.8% of the cytosine residues in M. hyorhinis DNA were methylated also. Analysis of cell culture DNA for the presence of m6Ade as a means for detection of contamination by mycoplasmas, and the phylogenetic implications of the finding of methylated bases in mycoplasmal DNAs are discussed.

MeSH Terms
5-Methylcytosine Acholeplasma laidlawii/metabolism Adenine/analogs & derivatives,metabolism Base Composition Cytosine/analogs & derivatives,metabolism DNA, Bacterial/metabolism Methylation Mycoplasma/metabolism
Chemicals
DNA, Bacterial 5-Methylcytosine Cytosine Adenine 6-methyladenine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Razin A
Razin S
References (10)
10 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.6.1383
Published
1980-03-25
Pages
1383-90
Language
English
Region
England
NLM ID
0411011
PMCID
PMC323998
Subset
IM
Grants
NIGMS NIH HHS · GM 20483 · United States
External Links
PubMed source DOI: 10.1093/nar/8.6.1383 PMC Full Text View journal details
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