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PMID: 7436883 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A rapid and efficient procedure for the removal of nonviable cells from suspension culture of lymphoid cell lines.

The Australian journal of experimental biology and medical science ·Vol. 58 ·No. 4 ·1980-08-00 ·Pages 373-6

Hurrell JG, Black PH, Haber E, Zurawski VR

Abstract

The selective removal of nonviable cells from suspension cultures of lymphoid cell lines by buoyant density centrifugation at 400 x g on a Ficoll-Hypaque (FH) cushion is described. Viable cells collect at the medium-FH interface whereas nonviable cells pellet. This method has proven useful in separating viable from nonviable cells with human lymphoblastoid cell lines, rabbit lymphoid cell lines and mouse myeloma and hybridoma cell lines.

MeSH Terms
Animals Cell Line Cell Separation Cell Survival Humans Lymphoid Tissue Mice Rabbits
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hurrell J G
Black P H
Haber E
Zurawski V R
Article Info
Journal
The Australian journal of experimental biology and medical science
Abbr.
Aust J Exp Biol Med Sci
ISSN
0004-945X
DOI
10.1038/icb.1980.36
Published
1980-08-00
Pages
373-6
Language
English
Region
Australia
NLM ID
0416662
Subset
IM
Grants
NCI NIH HHS · CA-10126 · United States
NHLBI NIH HHS · HL-19259 · United States
External Links
PubMed source DOI: 10.1038/icb.1980.36
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