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PMID: 7438884 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequences translated by Balbiani ring 75S RNA in vitro are present in giant secretory protein from Chironomus tentans.

Chromosoma ·Vol. 81 ·No. 1 ·1980-00-00 ·Pages 101-13

Rydlander L, Pigon A, Edström JE

Abstract

The 75S RNA originating in the large Balbiani rings 1 and 2 (BRI and 2) was isolated and used for in vitro translation in the mRNA dependent reticulocyte lystate. Conditions (K+-concentration, temperature, time etc). were optimized for obtaining translation products of maximal size. Polypeptide chains up to about 500,000 D were obtained but no complete translation products. Trypic fingerprints were performed on the in vitro products as well as on the secretory protein components nos. I and II+III labelled with 35S-methionine. There was a large degree of correspondence between the fingerprint of the in vitro product and that of component I but less to that of component II+III. The results suggest that 75S RNA with an origin in the BR1 and BR2 codes for the giant secretory protein component I.

MeSH Terms
Amino Acid Sequence Animals Chironomidae/genetics Diptera/genetics Genes Protein Biosynthesis RNA, Messenger/genetics RNA, Ribosomal/genetics Salivary Proteins and Peptides/analysis
Chemicals
RNA, Messenger RNA, Ribosomal Salivary Proteins and Peptides
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rydlander L
Pigon A
Edström J E
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25 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00292425
Published
1980-00-00
Pages
101-13
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00292425 View journal details
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