Absence of satellite DNA synthesis during meiotic prophase in mouse and human spermatocytes.
Mouse spermatocytes were labelled in situ with 3H-thymidine at successive stages of meiosis. Isolated mouse as well as human spermatocytes were similarly labelled under in vitro conditions. DNA synthesis was followed either by tracking radioactivities in Cs2SO4 gradients or by measuring reassociation kinetics. Mouse satellite DNA and the 3 satellites of human DNA are labelled during S-phase but not during pachytene. In the mouse genome, there is a preferential labelling of regions containing foldbacks (human spermatocytes were not analyzed in this respect). The absence of detectable pachytene synthesis in satellite DNA is consistent with genetic evidence on the absence of crossing-over in constitutive heterochromatin.
18 references, click to expand
-
Some properties of the single strands isolated from the DNA of the nuclear satellite of the mouse (Mus musculus).J Mol Biol. 1969 Mar 28;40(3):423-43 PMID: 5364715
-
Biochemistry of meiosis.Philos Trans R Soc Lond B Biol Sci. 1977 Mar 21;277(955):277-94 PMID: 16291
-
Spermatogenic cells of the prepuberal mouse. Isolation and morphological characterization.J Cell Biol. 1977 Jul;74(1):68-85 PMID: 874003
-
On the mechanism of chromatin loss induced by the B chromosome of maize.Genetics. 1972 May;71(1):73-96 PMID: 17248577
-
MOLECULAR SIZE AND CIRCULARITY OF DNA IN CELLS OF MAMMALS AND HIGHER PLANTS.Proc Natl Acad Sci U S A. 1965 Feb;53:356-62 PMID: 14294069
-
Telomeric satellite DNA functions in regulating recombination.Chromosoma. 1976 Jun 30;56(2):143-67 PMID: 976019
-
Meiotic crossing-over in lily and mouse.Nature. 1977 Sep 15;269(5625):240-2 PMID: 593319
-
Renaturation properties and localization in heterochromatin of human satellite DNA's.Biochim Biophys Acta. 1971 Nov 19;247(4):528-34 PMID: 5141664
-
Biochemical analysis of meiosis in the male mouse. II. DNA metabolism at pachytene.Chromosoma. 1977 Jul 8;62(3):255-68 PMID: 885031
-
A possible effect of heterochromatin on chromosome pairing.Proc Natl Acad Sci U S A. 1974 Jul;71(7):2787-90 PMID: 4527611
-
Genetic studies on heterochromatin in Drosophila melanogaster and their implications for the functions of satellite DNA.Chromosoma. 1978 Mar 22;66(1):71-98 PMID: 416935
-
Polymerase and kinase activities in relation to RNA synthesis during meiosis.Protoplasma. 1965;60(2):218-32 PMID: 5886480
-
The organization of DNA sequences in the mouse genome.Chromosoma. 1977 May 23;61(3):215-26 PMID: 872701
-
An electron microscopic study of mouse foldback DNA.Cell. 1975 Aug;5(4):429-46 PMID: 1157097
-
The modification of crossing over in maize by extraneous chromosomal elements.Theor Appl Genet. 1973 Jan;43(3-4):196-202 PMID: 24424983
-
DIFFERENCE IN THE BEHAVIOR OF EU- AND HETERO-CHROMATIN: CROSSING-OVER.Nature. 1965 Feb 13;205:725-6 PMID: 14287435
-
Biochemical analysis of meiosis in the male mouse. I. Separation of DNA labelling of specific spermatogenic stages.Chromosoma. 1977 Jul 8;62(3):243-53 PMID: 885030
-
Fractionation of mouse DNA in preparative Ag+ -Cs2SO4 gradients.Biochim Biophys Acta. 1973 Oct 26;324(3):309-19 PMID: 4796961
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