The investigation of idiopathic infertility by in vitro fertilization.

Fertility and sterility ·Vol. 34 ·No. 5 ·1980-11-00 ·Pages 431-8

Trounson AO, Leeton JF, Wood C, Webb J, Kovacs G

Abstract

The results of in vitro fertilization of mature oocytes obtained at diagnostic laparoscopy from patients with both known and unknown clinical causes of infertility are presented. A simple regimen involving the use of clomiphene and human chorionic gonadotropin which enabled laparoscopy to coincide with available surgical lists resulted in the recovery of mature oocytes from 15 of the 18 patients treated. Apparently normal embryos developed from five of six oocytes recovered from patients who had blocked fallopian tubes or who were being investigated after 8 to 12 months of artificial insemination by donor. No normal embryos developed from oocytes from nine patients who had sustained infertility of unknown cause for 2 years or more. When this latter group was compared with other groups a high rate of fertilization failure was noted, and severe polyspermy developed in two cases. The results are discussed in relation to the diagnosis of infertility and the management of infertile patients.

Keywords

Artificial Insemination Biology Clinical Research Clomiphene--administraction and dosage Endocrine System Examinations And Diagnoses Family Planning Fertility Agents Fertilization Gonadotropins Gonadotropins Chorionic--administraction and dosage Hormones In Vitro Infertility Physiology Reproduction Reproductive Control Agents Research Methodology

MeSH Terms

Embryo, Mammalian/physiology,physiopathology Fallopian Tubes/physiopathology Female Fertilization in Vitro Humans Infertility, Female/classification,etiology Insemination, Artificial, Heterologous Oocytes Pregnancy

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Trounson A O

Leeton J F

Wood C

Webb J

Kovacs G

Other Abstracts

eng

This study examines the use of in vitro fertilization as a diagnostic procedure for patients with long-term infertility of unknown cause (idiopathic infertility). 18 infertile patients participated in the study and included: 1) 5 patients with occluded fallopian tubes who served as controls

2) 3 patients who failed to become pregnant after 8 to 12 months of AID (artificial insemination by donor)

and 3) 10 patients who had not conceived in spite of 2 years of regular unprotected coitus (those with idiopathic infertility). Clomiphene citrate and human chorionic gonadotropins (hCG) were administered to control follicular development and maturation to coincide with time of laparoscopy. 25 mature oocytes were extracted from 15 out of the 18 patients at laparoscopy. In vitro fertilization was performed by adding washed spermatozoa to culture tube containing oocyte-cumulus mass and then incubating the culture. Embryo development occurred in the oocytes of 5 of 6 patients who had blocked fallopian tubes and who had AID. No normal embryos developed in the oocytes of 9 patients with idiopathic infertility. Furthermore, fertilization failures and oocyte abnormalities were higher in this group than in the other 2 groups. Clinical recommendations for managing the 3 groups of infertile patients were discussed. Further research should be done on the long-term infertility of unknown cause.

Article Info

Journal

Fertility and sterility

Abbr.

Fertil Steril

ISSN

0015-0282

DOI

10.1016/s0015-0282(16)45132-0

Published

1980-11-00

Pages

431-8

Language

English

Region

United States

NLM ID

0372772

Subset

External Links

PubMed source DOI: 10.1016/s0015-0282(16)45132-0 View journal details

Analysis Services

The investigation of idiopathic infertility by in vitro fertilization.

Contact

WeChat Official Account