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PMID: 74420 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of an endogenous type C RNA virus of mink (Mv1Lu) cells.

Journal of virology ·Vol. 25 ·No. 1 ·1978-01-00 ·Pages 129-37

Barbacid M, Tronick SR, Aaronson SA

Abstract

Cells of the established MvlLu mink line spontaneously released a reverse transcriptase-containing virus after long-term passage in tissue culture. By molecular hybridization, DNA of normal mink cells was found to possess extensive nucleotide sequence homology with a reverse-transcription product of the viral genome, demonstrating that the new isolate was an endogenous virus of mink origin. The mink virus shared antigenic determinants with the major structural proteins of known mammalian type C viruses. Double-antibody competition radioimmunoassays were developed by utilizing the purified major structural protein, p30, of the mink endogenous virus. The virus was shown to possess antigenic determinants unique from those of other known mammalian type C viruses. It exhibited a higher degree of immunological cross-reactivity with endogenous rat type C and horizontally transmitted feline leukemia viruses than with other mammalian type C viruses tested. The finding that mink cells can remain nonvirus producing for many cell generations argues that there normally exists some cellular restriction to endogenous virus expression in this species.

MeSH Terms
Animals Base Sequence Cell Line DNA/analysis DNA, Viral/analysis Epitopes Mink Nucleic Acid Conformation Nucleotides/analysis RNA Viruses Retroviridae/analysis,immunology,isolation & purification Viral Proteins/analysis,immunology
Chemicals
DNA, Viral Epitopes Nucleotides Viral Proteins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Barbacid M
Tronick S R
Aaronson S A
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44 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.25.1.129-137.1978
Published
1978-01-00
Pages
129-37
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353909
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.25.1.129-137.1978 PMC Full Text View journal details
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