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PMID: 7443546 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of the RNA region transferred from a representative primer, beta-globin mRNA, to influenza mRNA during in vitro transcription.

Nucleic acids research ·Vol. 8 ·No. 5 ·1980-03-11 ·Pages 925-42

Robertson HD, Dickson E, Plotch SJ, Krug RM

Abstract

Capped eukaryotic mRNAs strongly stimulate influenza viral RNA transcription in vitro and donate their cap and also additional nucleotides to the viral transcripts (1). To identify which bases of a given primer mRNA are transferred, we synthesized influenza viral mRNA using a primer rabbit globin mRNA (enriched in beta-globin mRNA) which had been labeled in vitro to high specific activity with 125I. We show that during transcription the same 125I-labeled oligonucleotides were transferred to the 5' termini of each of the eight viral mRNA segments. The predominant sequence, representing 75 percent of the transferred oligonucleotides, was identical to the first 13 nucleotides at the 5' end of beta-globin mRNA (m7G5'ppp5'm6AmC(m)ACUUGCUUUUG). Because only the C-residues are labeled with 125I, these results indicate that either the first 12, 13 or 14 5' terminal bases of beta-globin mRNA were transferred to the viral mRNAs. 125I-labeled oligonucleotides recovered from the viral mRNA in minor yields indicated that shorter 5' terminal pieces of beta-globin mRNA were sometimes transferred and that G was probably the first base inserted by transcription.

MeSH Terms
Animals Base Sequence Globins/genetics In Vitro Techniques Oligonucleotides/metabolism Orthomyxoviridae/genetics,metabolism RNA Caps/metabolism RNA, Messenger/genetics,metabolism RNA, Viral/biosynthesis,genetics Rabbits Transcription, Genetic
Chemicals
Oligonucleotides RNA Caps RNA, Messenger RNA, Viral Globins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Robertson H D
Dickson E
Plotch S J
Krug R M
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29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.5.925
Published
1980-03-11
Pages
925-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC323963
Subset
IM
Grants
NIAID NIH HHS · AI 11772 · United States
NCI NIH HHS · CA08748 · United States
External Links
PubMed source DOI: 10.1093/nar/8.5.925 PMC Full Text View journal details
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