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PMID: 7465425 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification and partial characterization of a DNA polymerase alpha species from calf thymus.

Nucleic acids research ·Vol. 8 ·No. 23 ·1980-12-11 ·Pages 5703-14

Grosse F, Krauss G

Abstract

We have purified a DNA polymerase alpha species from calf thymus to near homogeneity. The enzyme sediments at 5.7 S and contains two polypeptides of 123000 and 134000 daltons in about equimolar ratio. The enzyme is inhibited by aphidicolin and N-ethylmaleimide, and retains its activity in buffers containing moderate salt conditions. Activated DNA is a better substrate than poly-(dA) . (dT) 10.

MeSH Terms
Ampholyte Mixtures/pharmacology Animals Cattle DNA Polymerase II/antagonists & inhibitors,isolation & purification,metabolism DNA-Directed DNA Polymerase/isolation & purification Ethylmaleimide/pharmacology Molecular Weight Poly dA-dT Protein Conformation Substrate Specificity Thymus Gland/enzymology
Chemicals
Ampholyte Mixtures Poly dA-dT DNA Polymerase II DNA-Directed DNA Polymerase Ethylmaleimide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Grosse F
Krauss G
References (18)
18 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.23.5703
Published
1980-12-11
Pages
5703-14
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324335
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.23.5703 PMC Full Text View journal details
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