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PMID: 7474149 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The S2 subunit of the murine coronavirus spike protein is not involved in receptor binding.

Journal of virology ·Vol. 69 ·No. 11 ·1995-11-00 ·Pages 7260-3

Taguchi F

Abstract

The receptor-binding capacity of the S2 subunit of the murine coronavirus S protein was examined by testing the inhibition of virus-receptor binding. Sp-4 virus and S1N(330), which consists of the N-terminal 330 amino acids of the S1 protein, both of which exhibited receptor-binding capacity, were able to prevent the binding of cl-2 virus to the receptor, while the mutant protein S1N(330)-159, which failed to bind to the receptor protein, and S2TM-, which lacks the transmembrane and cytoplasmic domains normally existing in the S2, were unable to prevent the binding of cl-2. By using cultured DBT cells, it was revealed that the infection of cells by cl-2 virus was significantly inhibited by S1N(330) but not by S2TM-. These results indicate that the S2 protein is not involved in the receptor binding of murine coronaviruses.

MeSH Terms
Animals Base Sequence Binding Sites Blotting, Western Cell Membrane/physiology Coronavirus/physiology DNA Primers Macromolecular Substances Membrane Glycoproteins/antagonists & inhibitors,biosynthesis,isolation & purification,metabolism Mice Molecular Sequence Data Mutagenesis Receptors, Coronavirus Receptors, Virus/antagonists & inhibitors,metabolism Recombinant Proteins/biosynthesis,isolation & purification,metabolism Sequence Deletion Spike Glycoprotein, Coronavirus Transfection Viral Envelope Proteins/biosynthesis,isolation & purification,metabolism
Chemicals
DNA Primers Macromolecular Substances Membrane Glycoproteins Receptors, Coronavirus Receptors, Virus Recombinant Proteins Spike Glycoprotein, Coronavirus Viral Envelope Proteins spike glycoprotein, SARS-CoV spike protein, mouse hepatitis virus
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Taguchi F
National Institute of Neuroscience, NCNP, Tokyo, Japan.
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.69.11.7260-7263.1995
Published
1995-11-00
Pages
7260-3
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC189649
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.69.11.7260-7263.1995 PMC Full Text View journal details
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