Home LiteratureArticle Details

PMID: 7477293 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mechanosensory signalling in C. elegans mediated by the GLR-1 glutamate receptor.

Nature ·Vol. 378 ·No. 6552 ·1995-11-02 ·Pages 78-81

Maricq AV, Peckol E, Driscoll M, Bargmann CI

Abstract

NEURONAL signalling across synapses involves activation of many neurotransmitter receptors on postsynaptic cells. glr-1 encodes a potential glutamate receptor in the nematode Caenorhabditis elegans which is most similar to vertebrae AMPA-type ionotropic glutamate receptors. glr-1 is expressed in motor neurons and interneurons, including interneurons implicated in the control of locomotion. Here we investigate the contribution of glr-1 to the normal signalling of these neurons, by generating a deletion mutation in glr-1. We find that mutant worms are deficient in their ability to withdraw backwards when mechanically stimulated, but they withdraw normally in response to chemical repellents. The ASH sensory neurons mediate withdrawal responses both to mechanical stimuli and to repellents, and ASH makes chemical synapses with glr-1-expressing interneurons. Our results suggest that postsynaptic interneurons use different neurotransmitter receptors to process two sensory stimuli detected by one sensory neuron.

MeSH Terms

Amino Acid Sequence Animals Animals, Genetically Modified Base Sequence Caenorhabditis elegans/genetics,metabolism Caenorhabditis elegans Proteins DNA Primers Interneurons/metabolism Membrane Proteins/genetics,metabolism Molecular Sequence Data Motor Neurons/metabolism Mutagenesis, Insertional Nerve Tissue Proteins/genetics,metabolism Neurons/metabolism Polymerase Chain Reaction Receptors, AMPA Receptors, Glutamate/genetics,metabolism Sequence Deletion Signal Transduction Synapses/physiology

Chemicals

Caenorhabditis elegans Proteins DNA Primers Membrane Proteins Nerve Tissue Proteins Receptors, AMPA Receptors, Glutamate glr-1 protein, C elegans

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Maricq A V

Howard Hughes Medical Institute, Department of Anatomy, University of California, San Francisco 94143-0452, USA.

Peckol E

Driscoll M

Bargmann C I

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/378078a0

Published

1995-11-02

Pages

78-81

Language

English

Region

England

NLM ID

0410462

Subset

Databases

GENBANK

D13213, M36418, M36419, M36420, M36421, M73271, M91561, M91562, M91563, M97192, U34661, X59996, X60086, X63255, X71790, Z11581, Z11713, Z11715, Z11716, Z17238, Z17239

Corrections

ErratumIn

External Links

PubMed source DOI: 10.1038/378078a0 View journal details

Analysis Services

Mechanosensory signalling in C. elegans mediated by the GLR-1 glutamate receptor.

Contact

WeChat Official Account