Mutation detection using immobilized mismatch binding protein (MutS).

Nucleic acids research ·Vol. 23 ·No. 19 ·1995-10-11 ·Pages 3944-8

Wagner R, Debbie P, Radman M

Abstract

An accurate and highly sensitive mutation detection assay has been developed. The assay is based on the detection of mispaired and unpaired bases by immobilized mismatch binding protein (Escherichia coli MutS). The assay can detect most mismatches and all single base substitution mutations, as well as small addition or deletion mutations. The assay is simple to use and does not require the use of either radioactivity or gel electrophoresis.

MeSH Terms

Adenosine Triphosphatases Anemia, Sickle Cell/genetics Bacterial Proteins Base Composition Base Sequence DNA Mutational Analysis/methods DNA Repair DNA-Binding Proteins Electrophoresis, Polyacrylamide Gel Escherichia coli Escherichia coli Proteins Globins/genetics Heterozygote Homozygote Humans Luminescent Measurements Molecular Sequence Data MutS DNA Mismatch-Binding Protein Nucleic Acid Heteroduplexes Polymerase Chain Reaction

Chemicals

Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins Nucleic Acid Heteroduplexes Globins Adenosine Triphosphatases MutS DNA Mismatch-Binding Protein MutS protein, E coli

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Wagner R

Genecheck Inc., Fort Collins, CO 80525, USA.

Debbie P

Radman M

References (12)

12 references, click to expand

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Structures of mismatched base pairs in DNA and their recognition by the Escherichia coli mismatch repair system.
EMBO J. 1986 Dec 20;5(13):3697-703 PMID: 2951250
Mispair specificity of methyl-directed DNA mismatch correction in vitro.
J Biol Chem. 1988 May 15;263(14):6829-35 PMID: 2834393
Repair of DNA heteroduplexes containing small heterologous sequences in Escherichia coli.
Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1730-4 PMID: 1542666
Mechanisms and biological effects of mismatch repair.
Annu Rev Genet. 1991;25:229-53 PMID: 1812808
Screening for mutations by enzyme mismatch cleavage with T4 endonuclease VII.
Proc Natl Acad Sci U S A. 1995 Jan 3;92(1):87-91 PMID: 7816853
Detection of single DNA base mutations with mismatch repair enzymes.
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Current methods of mutation detection.
Mutat Res. 1993 Jan;285(1):125-44 PMID: 7678126
The rapid detection of unknown mutations in nucleic acids.
Nat Genet. 1993 Oct;5(2):111-7 PMID: 8252035
Mutation detection by mismatch binding protein, MutS, in amplified DNA: application to the cystic fibrosis gene.
Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2674-8 PMID: 7511817
MutS binding protects heteroduplex DNA from exonuclease digestion in vitro: a simple method for detecting mutations.
Nucleic Acids Res. 1994 Jul 11;22(13):2710-1 PMID: 7518920
Human glucokinase gene: isolation, characterization, and identification of two missense mutations linked to early-onset non-insulin-dependent (type 2) diabetes mellitus.
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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/23.19.3944

Published

1995-10-11

Pages

3944-8

Language

English

Region

England

NLM ID

0411011

PMCID

PMC307314

Subset

External Links

PubMed source DOI: 10.1093/nar/23.19.3944 PMC Full Text View journal details

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