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PMID: 7480337 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Expression of an Arabidopsis potassium channel gene in guard cells.

Plant physiology ·Vol. 109 ·No. 2 ·1995-10-00 ·Pages 371-4

Nakamura RL, McKendree WL, Hirsch RE, Sedbrook JC, Gaber RF, Sussman MR

Abstract

The Arabidopsis thaliana KAT1 cDNA encodes a voltage-gated inward-rectifying K+ channel. A KAT1 genomic DNA clone was isolated and sequenced, and a 5' promoter and coding sequences containing eight introns were identified. Reporter gene analysis of transgenic plants containing the KAT1 promoter fused to bacterial beta-glucuronidase showed robust beta-glucuronidase activity primarily in guard cells.

MeSH Terms

Arabidopsis/cytology,genetics,metabolism Arabidopsis Proteins Base Sequence Cloning, Molecular DNA Primers DNA, Plant/metabolism Genes, Plant Molecular Sequence Data Plant Proteins/biosynthesis,genetics Plants, Genetically Modified Polymerase Chain Reaction Potassium Channels/biosynthesis,genetics Potassium Channels, Inwardly Rectifying Recombinant Proteins/biosynthesis Restriction Mapping

Chemicals

Arabidopsis Proteins DNA Primers DNA, Plant KAT1 protein, Arabidopsis Plant Proteins Potassium Channels Potassium Channels, Inwardly Rectifying Recombinant Proteins

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Nakamura R L

Department of Biochemistry, Northwestern University, Evanston, Illinois 60208, USA.

McKendree W L

Hirsch R E

Sedbrook J C

Gaber R F

Sussman M R

References (14)

14 references, click to expand

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Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.109.2.371

Published

1995-10-00

Pages

371-4

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC157599

Subset

Databases

GENBANK

U25088

External Links

PubMed source DOI: 10.1104/pp.109.2.371 PMC Full Text View journal details

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Expression of an Arabidopsis potassium channel gene in guard cells.

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