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PMID: 7480349 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A rapeseed cold-inducible transcript encodes a phosphoenolpyruvate carboxykinase.

Plant physiology ·Vol. 109 ·No. 2 ·1995-10-00 ·Pages 611-8

Sáez-Vásquez J, Raynal M, Delseny M

Abstract

We have isolated a clone corresponding to a new cold-regulated gene from a cDNA library made from rapeseed (Brassica napus cv Samourai) cold-acclimated etiolated seedlings. Sequence analysis and homology searches showed that this clone encodes a protein highly homologous to the ATP-dependent phosphoenolpyruvate carboxykinase (PEPCK; EC 4.1.1.49) from Saccharomyces cerevisiae, Trypanosoma, Rhizobium sp., and Escherichia coli; we refer to the B. napus clone as BnPEPCK. A potential ATP-binding site existing in all PEPCK proteins was also found in BnPEPCK. Although there was a basal expression of BnPEPCK in seedlings grown at control, room temperature, the steady-state level of the transcripts increased at 4 degrees C and decreased to normal levels when the seedlings were returned to control temperature (22 degrees C). Using antibodies made against a recombinant histidine-BnPEPCK fusion protein, we demonstrated that BnPEPCK protein level is correlated with the accumulation of the BnPEPCK transcript.

MeSH Terms
Acclimatization Animals Base Sequence Blotting, Northern Blotting, Southern Blotting, Western Brassica/enzymology Cold Temperature DNA Primers DNA, Plant/isolation & purification Escherichia coli/enzymology Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Molecular Sequence Data Oligonucleotide Probes Phosphoenolpyruvate Carboxykinase (GTP)/biosynthesis,isolation & purification Polymerase Chain Reaction RNA, Messenger/analysis,biosynthesis,isolation & purification RNA, Plant/analysis,biosynthesis,isolation & purification Recombinant Fusion Proteins/biosynthesis,isolation & purification Rhizobium/enzymology Saccharomyces cerevisiae/enzymology Sequence Homology, Amino Acid Transcription, Genetic Trypanosoma/enzymology
Chemicals
DNA Primers DNA, Plant Oligonucleotide Probes RNA, Messenger RNA, Plant Recombinant Fusion Proteins Phosphoenolpyruvate Carboxykinase (GTP)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sáez-Vásquez J
Laboratoire de Physiologie et Biologie Moléculaire des Plantes, Unité de Recherche Associée 565 du Centre National de la Recherche Scientifique, Université de Perpignan, France.
Raynal M
Delseny M
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.109.2.611
Published
1995-10-00
Pages
611-8
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC157627
Subset
IM
Databases
GENBANK
U21745
External Links
PubMed source DOI: 10.1104/pp.109.2.611 PMC Full Text View journal details
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