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PMID: 7500038 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ontogenic development and tissue distribution of V gamma 1-expressing gamma/delta T lymphocytes in normal mice.

The Journal of experimental medicine ·Vol. 182 ·No. 6 ·1995-12-01 ·Pages 1921-30

Pereira P, Gerber D, Huang SY, Tonegawa S

Abstract

A hamster monoclonal antibody (mAb) recognizing an epitope in the V gamma 1-J gamma 4-C gamma 4 chain of the gamma/delta T cell receptor has been generated. Using this mAb, we have quantitated the occurrence of V gamma 1-bearing gamma/delta T cells in the developing thymus and in the lymphoid organs and several epithelia of adult mice. The V gamma 1-expressing cells constitute a minor gamma/delta T cell subpopulation during fetal and early postnatal life, but they constitute a major population of gamma/delta T cells in the thymus and in the peripheral lymphoid organs in adult mice. In addition, we found that V gamma 1-bearing cells comprise a large proportion (15-60%) of the gamma/delta T cells present in the intestinal epithelium (i-IEL) in all strains of mice tested. V gamma 1+ i-IEL are present in athymic (nude) mice and in antigen-free mice, demonstrating that they can develop extrathymically and that their presence in the intestinal epithelium is independent of the antigenic load of the gut. Our results show that V gamma 1-bearing lymphocytes account for the largest population of gamma/delta T cells in the mouse. This population includes a thymus-dependent component that homes to the secondary lymphoid organs and a thymus-independent component that constitutes a major fraction of the gamma/delta i-IELs.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Base Sequence Cricetinae DNA Primers/chemistry Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor Immunity, Mucosal Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred DBA Mice, Nude Molecular Sequence Data Receptors, Antigen, T-Cell, gamma-delta/metabolism Spleen/embryology,growth & development,immunology T-Lymphocyte Subsets/immunology Thymus Gland/embryology,growth & development,immunology Tissue Distribution
Chemicals
Antibodies, Monoclonal DNA Primers Receptors, Antigen, T-Cell, gamma-delta
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pereira P
Unité d'Immunobiologie, Centre National de la Recherche Scientifique URA 1961, Institut Pasteur, Paris, France.
Gerber D
Huang S Y
Tonegawa S
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.182.6.1921
Published
1995-12-01
Pages
1921-30
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192273
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.182.6.1921 PMC Full Text View journal details
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