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PMID: 7504685 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of "Flexispira rappini" from laboratory mice.

Journal of clinical microbiology ·Vol. 31 ·No. 10 ·1993-10-00 ·Pages 2709-14

Schauer DB, Ghori N, Falkow S

Abstract

A bacterium with an unusual ultrastructure and possessing a fusiform protoplasmic cylinder, spiral periplasmic fibers, and bipolar tufts of sheathed flagella was identified in the intestinal mucosae of laboratory mice. The organism was cultured under microaerophilic conditions and was found to rapidly hydrolyze urea. On the basis of 16S rRNA gene sequence analysis, the organism was shown to be "Flexispira rappini." "F. rappini" is closely related to members of the genus Helicobacter and has been reported to be associated with human gastroenteritis and ovine abortion. "F. rappini" has not previously been observed in the gastrointestinal tracts of mice.

MeSH Terms
Animals Bacteria/isolation & purification,pathogenicity,ultrastructure Base Sequence Cloning, Molecular Female Helicobacter/isolation & purification Intestinal Mucosa/microbiology Mice/microbiology Molecular Sequence Data RNA, Bacterial/chemistry,genetics RNA, Ribosomal, 16S/chemistry,genetics
Chemicals
RNA, Bacterial RNA, Ribosomal, 16S
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schauer D B
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402.
Ghori N
Falkow S
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.31.10.2709-2714.1993
Published
1993-10-00
Pages
2709-14
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC265986
Subset
IM
Grants
NIDDK NIH HHS · DK38707-06 · United States
Databases
GENBANK
L12765, M80205, M88137, M88138
External Links
PubMed source DOI: 10.1128/jcm.31.10.2709-2714.1993 PMC Full Text View journal details
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