Immunocytochemical evidence that the beta-protein precursor is an integral component of neurofibrillary tangles of Alzheimer's disease.

The American journal of pathology ·Vol. 143 ·No. 6 ·1993-12-00 ·Pages 1586-93

Perry G, Richey PL, Siedlak SL, Smith MA, Mulvihill P, DeWitt DA, Barnett J, Greenberg BD, Kalaria RN

Abstract

Amyloid beta (A beta) immunoreactivity has been demonstrated in all extracellular neurofibrillary tangles (E-NFT) and most intraneuronal neurofibrillary tangles (I-NFT). We undertook this immunocytochemical study to understand the relationship between A beta immunoreactivity localized in NFT and beta-protein precursor (beta PP). We found epitopes of amino-, mid-, and carboxyl-terminal domains of beta PP in I-NFT and the majority of E-NFT. NFT retained beta PP after ionic detergent extraction, demonstrating that beta PP is an integral component of NFT. Finding beta PP in regions of A beta immunoreactivity raises the possibility that beta PP or its fragments associate with amyloid, and that the stability of A beta is responsible for its dominance in amyloid deposits.

MeSH Terms

Aged Aged, 80 and over Alzheimer Disease/metabolism,pathology Amyloid beta-Protein Precursor/analysis,metabolism Epitopes Humans Immunohistochemistry Neurofibrillary Tangles/chemistry,pathology,ultrastructure

Chemicals

Amyloid beta-Protein Precursor Epitopes

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Perry G

Division of Neuropathology, Case Western Reserve University, Cleveland, Ohio 44106-4907.

Richey P L

Siedlak S L

Smith M A

Mulvihill P

DeWitt D A

Barnett J

Greenberg B D

Kalaria R N

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Article Info

Journal

The American journal of pathology

Abbr.

Am J Pathol

ISSN

0002-9440

Published

1993-12-00

Pages

1586-93

Language

English

Region

United States

NLM ID

0370502

PMCID

PMC1887275

Subset

External Links

PubMed source PMC Full Text View journal details

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Immunocytochemical evidence that the beta-protein precursor is an integral component of neurofibrillary tangles of Alzheimer's disease.

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