Home LiteratureArticle Details
PMID: 7505262 Published · ppublish English Comparative Study Journal Article

Cloning and nucleotide sequence analysis of psaA, the Streptococcus pneumoniae gene encoding a 37-kilodalton protein homologous to previously reported Streptococcus sp. adhesins.

Infection and immunity ·Vol. 62 ·No. 1 ·1994-01-00 ·Pages 319-24

Sampson JS, O'Connor SP, Stinson AR, Tharpe JA, Russell H

Abstract

Gene psaA, which encodes the Streptococcus pneumoniae 37-kDa protein, was cloned in Escherichia coli, and its complete nucleotide sequence was determined. Analysis of the sequence of the 2.4-kb cloned fragment revealed three open reading frames (ORFs). ORF2, which is 933 bp long, was identified as psaA. The two other ORFs identified flank psaA. ORF1, located upstream of psaA, is 836 nucleotides long and encodes a protein with a calculated molecular mass of 29,843 Da. The sequence for ORF3, located downstream of psaA, was only partially determined. Northern (RNA) blot analysis of pneumococcal RNA suggests that psaA is transcribed as part of a polycistronic message. Analysis of the primary structure of the protein encoded by this gene indicated significant similarity to two previously reported streptococcal proteins, SsaB (80% similarity) and FimA (92.3% similarity), from S. sanguis and S. parasanguis, respectively. These two homologous proteins have been shown to be associated with bacterial adhesion, and the possibility of a similar role for PsaA is hypothesized.

Related Genes
fimA psaA ssaB
MeSH Terms
Adhesins, Bacterial Amino Acid Sequence Bacterial Adhesion Bacterial Proteins/genetics Base Sequence Carrier Proteins Cloning, Molecular Gene Expression Genes, Bacterial Lipoproteins Membrane Transport Proteins Molecular Sequence Data RNA, Bacterial/genetics RNA, Messenger/genetics Sequence Alignment Sequence Homology, Amino Acid Streptococcus pneumoniae/immunology
Chemicals
Adhesins, Bacterial Bacterial Proteins Carrier Proteins Lipoproteins Membrane Transport Proteins PsaA protein, Streptococcus RNA, Bacterial RNA, Messenger
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sampson J S
Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
O'Connor S P
Stinson A R
Tharpe J A
Russell H
References (40)
40 references, click to expand
  1. Role of interbacterial adherence in colonization of the oral cavities of gnotobiotic rats infected with Streptococcus mutans and Veillonella alcalescens.
    Infect Immun. 1981 Aug;33(2):467-72 PMID: 7275312
  2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  3. Promoter for a developmentally regulated gene in Bacillus subtilis.
    Cell. 1981 Sep;25(3):783-91 PMID: 6269757
  4. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  5. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis.
    Mol Gen Genet. 1982;186(3):339-46 PMID: 6181373
  6. Patterns of amino acids near signal-sequence cleavage sites.
    Eur J Biochem. 1983 Jun 1;133(1):17-21 PMID: 6852022
  7. Molecular cloning of the temperature-inducible outer membrane protein 1 of Yersinia pseudotuberculosis.
    Infect Immun. 1984 Jan;43(1):72-8 PMID: 6317574
  8. Adherence of Streptococcus sanguis to saliva-coated hydroxyapatite: evidence for two binding sites.
    Infect Immun. 1984 Feb;43(2):656-63 PMID: 6319287
  9. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  10. Surface properties of Streptococcus sanguis FW213 mutants nonadherent to saliva-coated hydroxyapatite.
    Infect Immun. 1985 Mar;47(3):752-9 PMID: 2857684
  11. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  12. Antibodies that bind to fimbriae block adhesion of Streptococcus sanguis to saliva-coated hydroxyapatite.
    Infect Immun. 1985 Jun;48(3):617-24 PMID: 2860066
  13. Cell surface components of Streptococcus sanguis: relationship to aggregation, adherence, and hydrophobicity.
    J Bacteriol. 1985 Oct;164(1):255-62 PMID: 4044521
  14. Bacteremia in Charleston County, South Carolina.
    Am J Epidemiol. 1986 Jan;123(1):128-36 PMID: 3940431
  15. Biogenesis of lipoproteins in bacteria.
    Curr Top Microbiol Immunol. 1986;125:127-57 PMID: 3527576
  16. Identification and preliminary characterization of a Streptococcus sanguis fibrillar glycoprotein.
    J Bacteriol. 1987 Jan;169(1):164-71 PMID: 3793716
  17. Cloning of Streptococcus pneumoniae DNA fragments in Escherichia coli requires vectors protected by strong transcriptional terminators.
    Gene. 1987;55(2-3):179-87 PMID: 3311881
  18. Purified 60-kilodalton Legionella protein antigen with Legionella-specific and nonspecific epitopes.
    J Clin Microbiol. 1987 Nov;25(11):2080-4 PMID: 2447117
  19. The GenBank genetic sequence data bank.
    Nucleic Acids Res. 1988 Mar 11;16(5):1861-3 PMID: 3353225
  20. Cloning of a Streptococcus sanguis adhesin which mediates binding to saliva-coated hydroxyapatite.
    Infect Immun. 1988 May;56(5):1150-7 PMID: 3356463
  21. Molecular characterization of a surface protein antigen gene from serotype c Streptococcus mutans, implicated in dental caries.
    Mol Microbiol. 1989 May;3(5):673-8 PMID: 2761390
  22. Nucleotide sequence analysis of a type 1 fimbrial gene of Streptococcus sanguis FW213.
    Infect Immun. 1989 Nov;57(11):3527-33 PMID: 2572555
  23. Streptococcus pneumoniae possesses canonical Escherichia coli (sigma 70) promoters.
    Mol Microbiol. 1990 Jul;4(7):1143-52 PMID: 2233251
  24. Nucleotide sequence of a gene coding for a saliva-binding protein (SsaB) from Streptococcus sanguis 12 and possible role of the protein in coaggregation with actinomyces.
    Infect Immun. 1991 Mar;59(3):1093-9 PMID: 1671775
  25. Sequence analysis of the cloned streptococcal cell surface antigen I/II.
    FEBS Lett. 1989 Nov 20;258(1):127-32 PMID: 2687020
  26. Streptococcus parasanguis sp. nov., an atypical viridans Streptococcus from human clinical specimens.
    FEMS Microbiol Lett. 1990 Mar 1;56(1-2):115-21 PMID: 1692001
  27. Streptococcal-host interactions. Structural and functional analysis of a Streptococcus sanguis receptor for a human salivary glycoprotein.
    J Biol Chem. 1990 May 5;265(13):7120-6 PMID: 2185241
  28. Partial 16S rRNA primary structure of five Actinomyces species: phylogenetic implications and development of an Actinomyces israelii-specific oligonucleotide probe.
    J Gen Microbiol. 1990 Jan;136(1):37-43 PMID: 1693659
  29. Characterization of Streptococcus gordonii (S. sanguis) PK488 adhesin-mediated coaggregation with Actinomyces naeslundii PK606.
    Infect Immun. 1990 Sep;58(9):3064-72 PMID: 2387635
  30. Nucleotide sequence of htpB, the Legionella pneumophila gene encoding the 58-kilodalton (kDa) common antigen, formerly designated the 60-kDa common antigen.
    Infect Immun. 1990 Sep;58(9):3154-7 PMID: 2117582
  31. Bacterial adhesion: genetics, biogenesis, and role in pathogenesis of fimbrial adhesins of Escherichia coli.
    Rev Infect Dis. 1991 Jul-Aug;13(4):721-35 PMID: 1681580
  32. Sequence and structural analysis of surface protein antigen I/II (SpaA) of Streptococcus sobrinus.
    Infect Immun. 1991 Aug;59(8):2677-85 PMID: 1855987
  33. Monoclonal antibody recognizing a species-specific protein from Streptococcus pneumoniae.
    J Clin Microbiol. 1990 Oct;28(10):2191-5 PMID: 2229341
  34. Adherence, coaggregation, and hydrophobicity of Streptococcus gordonii associated with expression of cell surface lipoproteins.
    Infect Immun. 1992 Mar;60(3):1225-8 PMID: 1339408
  35. Saliva-binding protein (SsaB) from Streptococcus sanguis 12 is a lipoprotein.
    J Bacteriol. 1993 Jan;175(2):572-4 PMID: 8419308
  36. Cloning of the Streptococcus gordonii PK488 gene, encoding an adhesin which mediates coaggregation with Actinomyces naeslundii PK606.
    Infect Immun. 1993 Mar;61(3):981-7 PMID: 8432618
  37. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  38. Molecular weight determination of membrane protein and glycoprotein subunits by discontinuous gel electrophoresis in dodecyl sulfate.
    Methods Enzymol. 1974;32:92-102 PMID: 4444547
  39. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  40. Rapid and efficient cosmid cloning.
    Nucleic Acids Res. 1981 Jul 10;9(13):2989-98 PMID: 6269067
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.62.1.319-324.1994
Published
1994-01-00
Pages
319-24
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC186105
Subset
IM
Databases
GENBANK
L19055
External Links
PubMed source DOI: 10.1128/iai.62.1.319-324.1994 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2