Home LiteratureArticle Details
PMID: 7517129 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Quantification of methanogenic groups in anaerobic biological reactors by oligonucleotide probe hybridization.

Applied and environmental microbiology ·Vol. 60 ·No. 4 ·1994-04-00 ·Pages 1241-8

Raskin L, Poulsen LK, Noguera DR, Rittmann BE, Stahl DA

Abstract

The microbial community structure of anaerobic biological reactors was evaluated by using oligonucleotide probes complementary to conserved tracts of the 16S rRNAs of phylogenetically defined groups of methanogens. Phylogenetically defined groups of methanogens were quantified and visualized, respectively, by hybridization of 32P- and fluorescent-dye-labeled probes to the 16S rRNAs from samples taken from laboratory acetate-fed chemostats, laboratory municipal solid waste digestors, and full-scale sewage sludge digestors. Methanosarcina species, members of the order Methanobacteriales, and Methanosaeta species were the most abundant methanogens present in the chemostats, the solid-waste digestors, and the sewage sludge digestors, respectively.

MeSH Terms
Anaerobiosis Bacteriological Techniques/instrumentation DNA, Bacterial/analysis DNA, Ribosomal/analysis Euryarchaeota/classification,genetics,growth & development,isolation & purification,metabolism Nucleic Acid Hybridization Oligonucleotide Probes Population Dynamics RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Refuse Disposal/instrumentation Sewage/analysis
Chemicals
DNA, Bacterial DNA, Ribosomal Oligonucleotide Probes RNA, Bacterial RNA, Ribosomal, 16S Sewage
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Raskin L
Department of Civil Engineering, University of Illinois at Urbana-Champaign, 61801.
Poulsen L K
Noguera D R
Rittmann B E
Stahl D A
References (25)
25 references, click to expand
  1. Archaea in coastal marine environments.
    Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5685-9 PMID: 1608980
  2. Methanogenesis from sucrose by defined immobilized consortia.
    Appl Environ Microbiol. 1984 Jan;47(1):1-6 PMID: 16346452
  3. Energetics of Growth of a Defined Mixed Culture of Desulfovibrio vulgaris and Methanosarcina barkeri: Interspecies Hydrogen Transfer in Batch and Continuous Cultures.
    Appl Environ Microbiol. 1983 Nov;46(5):1152-6 PMID: 16346421
  4. Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms.
    Appl Environ Microbiol. 1993 May;59(5):1354-60 PMID: 7685999
  5. Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology.
    Appl Environ Microbiol. 1988 May;54(5):1079-84 PMID: 3389805
  6. Phylogenetic structure of the prokaryotic domain: the primary kingdoms.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):5088-90 PMID: 270744
  7. Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms.
    Appl Environ Microbiol. 1992 Feb;58(2):614-23 PMID: 1376982
  8. Sulfate reduction relative to methane production in high-rate anaerobic digestion: technical aspects.
    Appl Environ Microbiol. 1986 Mar;51(3):572-9 PMID: 16347018
  9. Isolation and Characterization of a Thermophilic Bacterium Which Oxidizes Acetate in Syntrophic Association with a Methanogen and Which Grows Acetogenically on H(2)-CO(2).
    Appl Environ Microbiol. 1988 Jan;54(1):124-129 PMID: 16347518
  10. Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells.
    Science. 1989 Mar 10;243(4896):1360-3 PMID: 2466341
  11. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology.
    J Bacteriol. 1990 Feb;172(2):762-70 PMID: 1688842
  12. Kinetics of Acetate Utilization by Two Thermophilic Acetotrophic Methanogens: Methanosarcina sp. Strain CALS-1 and Methanothrix sp. Strain CALS-1.
    Appl Environ Microbiol. 1989 Feb;55(2):488-91 PMID: 16347856
  13. Lamina, a novel multicellular form of Methanosarcina mazei S-6.
    J Bacteriol. 1992 Jan;174(1):309-14 PMID: 1370285
  14. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.
    Appl Environ Microbiol. 1990 Jun;56(6):1919-25 PMID: 2200342
  15. Occurrence of sulphate-reducing bacteria in human faeces and the relationship of dissimilatory sulphate reduction to methanogenesis in the large gut.
    J Appl Bacteriol. 1988 Aug;65(2):103-11 PMID: 3204069
  16. Immunochemical differences among Methanosarcina mazei S-6 morphologic forms.
    J Bacteriol. 1992 Jul;174(14):4683-8 PMID: 1624456
  17. Bacterial population development and chemical characteristics of refuse decomposition in a simulated sanitary landfill.
    Appl Environ Microbiol. 1989 Jan;55(1):55-65 PMID: 16347835
  18. Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens.
    Appl Environ Microbiol. 1994 Apr;60(4):1232-40 PMID: 7517128
  19. Novel major archaebacterial group from marine plankton.
    Nature. 1992 Mar 12;356(6365):148-9 PMID: 1545865
  20. Characterization of metabolic performance of methanogenic granules treating brewery wastewater: role of sulfate-reducing bacteria.
    Appl Environ Microbiol. 1991 Dec;57(12):3438-49 PMID: 1785921
  21. Control/promotion of the refuse methanogenic fermentation.
    Crit Rev Biotechnol. 1990;10(2):93-118 PMID: 2117998
  22. Monitoring the enrichment and isolation of sulfate-reducing bacteria by using oligonucleotide hybridization probes designed from environmentally derived 16S rRNA sequences.
    Appl Environ Microbiol. 1993 Mar;59(3):682-6 PMID: 7683181
  23. Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4576-9 PMID: 2112744
  24. Kinetics of the methanogenic fermentation of acetate.
    Appl Environ Microbiol. 1990 Oct;56(10):3158-63 PMID: 16348323
  25. Acetate as sole carbon and energy source for growth of methanosarcina strain 227.
    Appl Environ Microbiol. 1980 May;39(5):993-9 PMID: 16345576
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.60.4.1241-1248.1994
Published
1994-04-00
Pages
1241-8
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC201465
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.60.4.1241-1248.1994 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2