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PMID: 7518840 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Basic fibroblast growth factor improves myocardial function in chronically ischemic porcine hearts.

The Journal of clinical investigation ·Vol. 94 ·No. 2 ·1994-08-00 ·Pages 623-30

Harada K, Grossman W, Friedman M, Edelman ER, Prasad PV, Keighley CS, Manning WJ, Sellke FW, Simons M

Abstract

The effect of basic fibroblast growth factor (bFGF) administration on regional myocardial function and blood flow in chronically ischemic hearts was studied in 26 pigs instrumented with proximal circumflex coronary artery (LCX) ameroid constrictors. In 13 animals bFGF was administered extraluminally to the proximal left anterior descending (LAD) and LCX arteries with heparin-alginate beads and 13 other animal served as controls. bFGF-treated pigs showed a fourfold reduction in left ventricular infarct size compared to untreated controls (infarct size: 1.2 +/- 0.4% vs. 5.1 +/- 1.3% of LV mass, mean +/- SEM, P < 0.05). Percent fractional shortening (% FS) in the LCX area at rest was reduced compared with the LAD region in both bFGF and control pigs. However, there was better recovery in the LCX area after rapid pacing in bFGF-treated pigs (% FSLCX/% FSLAD, 22.9 +/- 7.3%-->30.5 +/- 8.5%, P < 0.05 vs. prepacing) than in controls (16.0 +/- 7.8%-->14.3 +/- 7.0%, P = NS). Furthermore, LV end-diastolic pressure rise with rapid pacing was less in bFGF-treated than control pigs (pre-pacing; pacing; post-pacing, 10 +/- 1; 17 +/- 3; 11 +/- 1* mmHg vs 10 +/- 1; 24 +/- 4; 15 +/- 1 mmHg, *P < 0.05 vs. control). Coronary blood flow in the LCX territory (normalized for LAD flow) was also better during pacing in bFGF-treated pigs than in controls. Thus, periadventitial administration of bFGF in a gradual coronary occlusion model in pigs results in improvement of coronary flow and reduction in infarct size in the compromised territory as well as in prevention of pacing-induced hemodynamic deterioration.

MeSH Terms
Animals Chronic Disease Coronary Circulation/drug effects Echocardiography Female Fibroblast Growth Factor 2/pharmacology Heart/drug effects,physiopathology Male Myocardial Ischemia/physiopathology Neovascularization, Pathologic/chemically induced Swine Ventricular Function, Left
Chemicals
Fibroblast Growth Factor 2
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Harada K
Charles A. Dana Research Institute, Boston, Massachusetts.
Grossman W
Friedman M
Edelman E R
Prasad P V
Keighley C S
Manning W J
Sellke F W
Simons M
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI117378
Published
1994-08-00
Pages
623-30
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC296139
Subset
IM
Grants
NHLBI NIH HHS · HL-44431 · United States
NHLBI NIH HHS · HL-46716 · United States
NIA NIH HHS · K12 AG00294 · United States
External Links
PubMed source DOI: 10.1172/JCI117378 PMC Full Text View journal details
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