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PMID: 7519776 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Assembly of synthetic cellulose I.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 16 ·1994-08-02 ·Pages 7425-9

Lee JH, Brown RM, Kuga S, Shoda S, Kobayashi S

Abstract

Cellulose microfibrils with an electron diffraction pattern characteristic of crystalline native cellulose I have been assembled abiotically by means of a cellulase-catalyzed polymerization of beta-cellobiosyl fluoride substrate monomer in acetonitrile/acetate buffer. Substantial purification of the Trichoderma viride cellulase enzyme was found to be essential for the formation of the synthetic cellulose I allomorph. Assembly of synthetic cellulose I appears to be a result of a micellar aggregation of the partially purified enzyme and the substrate in an organic/aqueous solvent system favoring the alignment of glucan chains with the same polarity and extended chain conformation, resulting in crystallization to form the metastable cellulose I allomorph.

MeSH Terms

Acetates Acetonitriles Buffers Carbohydrate Sequence Cellobiose/analogs & derivatives,metabolism Cellulase/isolation & purification,metabolism Cellulose/biosynthesis Cellulose 1,4-beta-Cellobiosidase Electrons Glycoside Hydrolases/metabolism Gold Colloid Microscopy, Electron Molecular Sequence Data Scattering, Radiation Staining and Labeling Trichoderma/enzymology

Chemicals

Acetates Acetonitriles Buffers Gold Colloid cellobiosyl fluoride Cellobiose Cellulose Glycoside Hydrolases Cellulase Cellulose 1,4-beta-Cellobiosidase acetonitrile

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Lee J H

Department of Botany, University of Texas, Austin 78713-7640.

Brown R M

Kuga S

Shoda S

Kobayashi S

References (10)

10 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.91.16.7425

Published

1994-08-02

Pages

7425-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC44413

Subset

Corrections

ErratumIn

External Links

PubMed source DOI: 10.1073/pnas.91.16.7425 PMC Full Text View journal details

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