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PMID: 7522321 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

P-selectin induces the expression of tissue factor on monocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 19 ·1994-09-13 ·Pages 8767-71

Celi A, Pellegrini G, Lorenzet R, De Blasi A, Ready N, Furie BC, Furie B

Abstract

P-selectin on activated platelets and stimulated endothelial cells mediates cell adhesion with monocytes and neutrophils. Since activated platelets induce tissue factor on mononuclear leukocytes, we examined the effect of P-selectin on the expression of tissue factor activity in monocytes. Purified P-selectin stimulated tissue factor expression on mononuclear leukocytes in a dose-dependent manner. Chinese hamster ovary (CHO) cells expressing P-selectin stimulated tissue factor procoagulant activity in purified monocytes, whereas untransfected CHO cells and CHO cells expressing E-selectin did not. Anti-P-selectin antibodies inhibited the effects of purified P-selectin and CHO cells expressing P-selectin on monocytes. Incubation of CHO cells expressing P-selectin with monocytes leads to the development of tissue factor mRNA in monocytes and to the expression of tissue factor antigen on the monocyte surface. These results indicate that P-selectin upregulates the expression of tissue factor on monocytes as well as mediates the binding of platelets and endothelial cells with monocytes and neutrophils. The binding of P-selectin to monocytes in the area of vascular injury may be a component of a mechanism that initiates thrombosis.

MeSH Terms
Cell Adhesion Molecules/pharmacology Cells, Cultured Dose-Response Relationship, Drug Gene Expression/drug effects Humans In Vitro Techniques Monocytes/metabolism P-Selectin Platelet Membrane Glycoproteins/pharmacology RNA, Messenger/genetics Thromboplastin/genetics,metabolism
Chemicals
Cell Adhesion Molecules P-Selectin Platelet Membrane Glycoproteins RNA, Messenger Thromboplastin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Celi A
Center for Hemostasis and Thrombosis Research, New England Medical Center, Boston, MA 02111.
Pellegrini G
Lorenzet R
De Blasi A
Ready N
Furie B C
Furie B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.19.8767
Published
1994-09-13
Pages
8767-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC44687
Subset
IM
Grants
NHLBI NIH HHS · HL42443 · United States
NHLBI NIH HHS · T32 HL07437 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.19.8767 PMC Full Text View journal details
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