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PMID: 7536617 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

CNS gene delivery by retrograde transport of recombinant replication-defective adenoviruses.

Gene therapy ·Vol. 2 ·No. 2 ·1995-03-00 ·Pages 132-7

Ghadge GD, Roos RP, Kang UJ, Wollmann R, Fishman PS, Kalynych AM, Barr E, Leiden JM

Abstract

The ability to program recombinant gene expression in specific sets of motor and sensory neurons would facilitate the treatment of a number of acquired and inherited central nervous system (CNS) diseases. In this report, we demonstrate that intramuscular injection of replication-defective recombinant adenovirus results in high-level recombinant gene expression, specifically in the CNS motor and sensory neurons that innervate the inoculated muscles. Neural expression of the recombinant genes results from virus transport into the CNS, presumably by retrograde axonal transport. This novel method of neural gene delivery may be of value in studies designed to improve understanding and treatment of inherited and acquired neurological diseases.

MeSH Terms

Adenoviridae/genetics,isolation & purification Afferent Pathways Animals Axonal Transport Base Sequence Brain Stem/virology Cerebral Ventricles/virology DNA, Recombinant/analysis DNA, Viral/analysis Defective Viruses/genetics Genetic Vectors/administration & dosage,pharmacokinetics Hindlimb/innervation Hypoglossal Nerve/virology Injections, Intramuscular Mice Mice, Inbred Strains Molecular Sequence Data Motor Neurons/virology Muscles/innervation,virology Neurons, Afferent/virology Polymerase Chain Reaction Recombinant Fusion Proteins/biosynthesis,genetics Spinal Cord/virology Tibial Nerve/virology Tongue/innervation Trigeminal Nerve/virology beta-Galactosidase/biosynthesis,genetics

Chemicals

DNA, Recombinant DNA, Viral Recombinant Fusion Proteins beta-Galactosidase

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Ghadge G D

Department of Neurology, University of Chicago School of Medicine, IL 60637.

Roos R P

Kang U J

Wollmann R

Fishman P S

Kalynych A M

Barr E

Leiden J M

Article Info

Journal

Gene therapy

Abbr.

Gene Ther

ISSN

0969-7128

Published

1995-03-00

Pages

132-7

Language

English

Region

England

NLM ID

9421525

Subset

External Links

PubMed source View journal details

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CNS gene delivery by retrograde transport of recombinant replication-defective adenoviruses.

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