Epidermal growth factor induces the tyrosine phosphorylation and nuclear translocation of Stat 5 in mouse liver.
Intraperitoneal injection of epidermal growth factor into mice results in the appearance of multiple tyrosine-phosphorylated proteins in liver nuclei within minutes after administration. We have previously identified three of these proteins as Stat 1 alpha, Stat 1 beta (p91, p84), and Stat 3 (p89). In the present report we demonstrate that Stat 5 (p92), the recently described prolactin inducible transcription factor detected in mammary glands, is the major tyrosine-phosphorylated protein translocated to the nucleus in mouse liver in response to epidermal growth factor. Furthermore, gel-shift analysis and affinity purification revealed that Stat 5, Stat 1 alpha, and Stat 1 beta specifically bind to the prolactin inducible element upstream of the beta-casein promoter.
14 references, click to expand
-
Growth factor receptor tyrosine kinases.Annu Rev Biochem. 1988;57:443-78 PMID: 3052279
-
Signal transduction by receptors with tyrosine kinase activity.Cell. 1990 Apr 20;61(2):203-12 PMID: 2158859
-
The epidermal growth factor. A review of structural and functional relationships in the normal organism and in cancer cells.Tumour Biol. 1990;11(5):229-61 PMID: 2203137
-
Metabolism and effects of epidermal growth factor and related growth factors in mammals.Endocr Rev. 1990 Aug;11(3):418-42 PMID: 2226349
-
Epidermal growth factor stimulates tyrosine phosphorylation in the neonatal mouse: association of a M(r) 55,000 substrate with the receptor.Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8477-81 PMID: 1382290
-
Induction by EGF and interferon-gamma of tyrosine phosphorylated DNA binding proteins in mouse liver nuclei.Science. 1993 Sep 24;261(5129):1733-6 PMID: 8378774
-
Receptors for epidermal growth factor and other polypeptide mitogens.Annu Rev Biochem. 1987;56:881-914 PMID: 3039909
-
Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6.Science. 1994 Apr 1;264(5155):95-8 PMID: 8140422
-
Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response.EMBO J. 1994 May 1;13(9):2182-91 PMID: 7514531
-
Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins.Science. 1994 Jun 3;264(5164):1415-21 PMID: 8197455
-
Epidermal growth factor and lipopolysaccharide activate Stat3 transcription factor in mouse liver.J Biol Chem. 1994 Sep 2;269(35):21933-5 PMID: 8071311
-
Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription.EMBO J. 1994 Sep 15;13(18):4361-9 PMID: 7925280
-
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
-
A common nuclear signal transduction pathway activated by growth factor and cytokine receptors.Science. 1993 Sep 24;261(5129):1739-44 PMID: 8397445
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
