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PMID: 7539049 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Functional properties of cardiac L-type calcium channels transiently expressed in HEK293 cells. Roles of alpha 1 and beta subunits.

The Journal of general physiology ·Vol. 105 ·No. 2 ·1995-02-00 ·Pages 289-305

Pérez-García MT, Kamp TJ, Marbán E

Abstract

The cardiac dihydropyridine-sensitive calcium channel was transiently expressed in HEK293 cells by transfecting the rabbit cardiac calcium channel alpha 1 subunit (alpha 1C) alone or in combination with the rabbit calcium channel beta subunit cloned from skeletal muscle. Transfection with alpha 1C alone leads to the expression of inward, voltage-activated, calcium or barium currents that exhibit dihydropyridine sensitivity and voltage- as well as calcium-dependent inactivation. Coexpression of the skeletal muscle beta subunit increases current density and the number of high-affinity dihydropyridine binding sites and also affects the macroscopic kinetics of the current. Recombinant alpha 1C beta channels exhibit a slowing of activation and a faster inactivation rate when either calcium or barium carries the charge. Our data suggest that both an increase in the number of channels as well as modulatory effects on gating underlie the modifications observed upon beta subunit coexpression.

MeSH Terms
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester/pharmacology Barium/pharmacokinetics Calcium/pharmacokinetics Calcium Channels/physiology Calcium Channels, L-Type Cells, Cultured Electrophysiology Humans Isradipine/pharmacology Kinetics Muscle Proteins/physiology Myocardium/chemistry Nitrendipine/pharmacology Time Factors Transfection
Chemicals
Calcium Channels Calcium Channels, L-Type Muscle Proteins Barium 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester Nitrendipine Calcium Isradipine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pérez-García M T
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Kamp T J
Marbán E
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46 references, click to expand
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.105.2.289
Published
1995-02-00
Pages
289-305
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2216941
Subset
IM
Grants
NHLBI NIH HHS · R01 HL 36957 · United States
External Links
PubMed source DOI: 10.1085/jgp.105.2.289 PMC Full Text View journal details
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