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PMID: 7540647 Published · ppublish English Comparative Study Journal Article

Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo.

The Journal of experimental medicine ·Vol. 182 ·No. 1 ·1995-07-01 ·Pages 155-62

Angiolillo AL, Sgadari C, Taub DD, Liao F, Farber JM, Maheshwari S, Kleinman HK, Reaman GH, Tosato G

Abstract

Human interferon-inducible protein 10 (IP-10), a member of the alpha chemokine family, inhibits bone marrow colony formation, has antitumor activity in vivo, is chemoattractant for human monocytes and T cells, and promotes T cell adhesion to endothelial cells. Here we report that IP-10 is a potent inhibitor of angiogenesis in vivo. IP-10 profoundly inhibited basic fibroblast growth factor-induced neovascularization of Matrigel (prepared by H. K. Kleinman) injected subcutaneously into athymic mice. In addition, IP-10, in a dose-dependent fashion, suppressed endothelial cell differentiation into tubular capillary structures in vitro. IP-10 had no effect on endothelial cell growth, attachment, and migration as assayed in vitro. These results document an important biological property of IP-10 and raise the possibility that IP-10 may participate in the regulation of angiogenesis during inflammation and tumorigenesis.

MeSH Terms
Amino Acid Sequence Animals Cell Adhesion/drug effects Cell Differentiation/drug effects Cell Division/drug effects Cell Movement/drug effects Cells, Cultured Chemokine CXCL10 Chemokines, CXC Collagen Cytokines/pharmacology,therapeutic use Drug Combinations Endothelium, Vascular/cytology,drug effects Female Fibroblast Growth Factor 2/antagonists & inhibitors,toxicity Humans Laminin Lung/blood supply Mice Mice, Inbred BALB C Mice, Nude Molecular Sequence Data Neovascularization, Pathologic/chemically induced,prevention & control Proteoglycans Umbilical Veins
Chemicals
Chemokine CXCL10 Chemokines, CXC Cytokines Drug Combinations Laminin Proteoglycans Fibroblast Growth Factor 2 matrigel Collagen
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Angiolillo A L
Department of Hematology/Oncology, Children's National Medical Center, Washington, DC 20010, USA.
Sgadari C
Taub D D
Liao F
Farber J M
Maheshwari S
Kleinman H K
Reaman G H
Tosato G
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.182.1.155
Published
1995-07-01
Pages
155-62
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192108
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.182.1.155 PMC Full Text View journal details
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