Long PCR-ribotyping of nontypeable Haemophilus influenzae.
PCR-ribotyping, a new typing method based on long PCR, has been developed for nontypeable Haemophilus influenzae (NTHi). Ribosomal operons of NTHi were amplified by long PCR and were found to be highly polymorphic for internal HaeIII sites. The technique was applied to 49 isolates previously subjected to conventional ribotyping, and the two methods showed a high level of concordance for serial isolates from individual subjects. PCR-ribotyping provides a powerful new typing tool for strain characterization in epidemiological investigations of NTHi.
13 references, click to expand
-
Partial duplication of the large ribosomal RNA sequence in an inverted repeat in circular mitochondrial DNA from Kloeckera africana. Implications for mechanisms of the petite mutation.J Mol Biol. 1981 Apr 15;147(3):399-415 PMID: 7198150
-
Evoked potentials: computer-automated threshold-tracking procedure using an objective detection criterion.Ear Hear. 1987 Jun;8(3):151-6 PMID: 3609510
-
Comparison of 16S rRNA sequences from the family Pasteurellaceae: phylogenetic relatedness by cluster analysis.J Gen Microbiol. 1988 Jul;134(7):1923-30 PMID: 2469775
-
Ribosomal RNA gene restriction patterns provide increased sensitivity for typing Salmonella typhi strains.J Infect Dis. 1989 Jul;160(1):145-9 PMID: 2471748
-
Characterization of noncapsulate Haemophilus influenzae by whole-cell polypeptide profiles, restriction endonuclease analysis, and rRNA gene restriction patterns.J Clin Microbiol. 1991 Feb;29(2):291-6 PMID: 1706727
-
Ribosomal DNA: molecular evolution and phylogenetic inference.Q Rev Biol. 1991 Dec;66(4):411-53 PMID: 1784710
-
Bacterial colonization of the nasopharynx predicts very early onset and persistence of otitis media in Australian aboriginal infants.Pediatr Infect Dis J. 1994 Nov;13(11):983-9 PMID: 7845752
-
Molecular epidemiology: application of contemporary techniques to the typing of microorganisms.Clin Infect Dis. 1993 Aug;17(2):153-62; quiz 163-4 PMID: 8399860
-
Polymerase chain reaction-based strain characterization of noncapsulate Haemophilus influenzae.J Clin Microbiol. 1993 Nov;31(11):2981-7 PMID: 8263183
-
PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2216-20 PMID: 8134376
-
Effective amplification of long targets from cloned inserts and human genomic DNA.Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5695-9 PMID: 8202550
-
Effects of thermocyclers and primers on the reproducibility of banding patterns in randomly amplified polymorphic DNA analysis.Mol Cell Probes. 1994 Apr;8(2):155-9 PMID: 7935514
-
Artifactual variation in randomly amplified polymorphic DNA banding patterns.Biotechniques. 1993 Feb;14(2):214-7 PMID: 8431284
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