Home LiteratureArticle Details
PMID: 7542800 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.

Science (New York, N.Y.) ·Vol. 269 ·No. 5223 ·1995-07-28 ·Pages 496-512

Fleischmann RD, Adams MD, White O, Clayton RA, Kirkness EF, Kerlavage AR, Bult CJ, Tomb JF, Dougherty BA, Merrick JM

Abstract

An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830,137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.

MeSH Terms
Bacterial Proteins/genetics Base Composition Base Sequence Chromosome Mapping/methods Chromosomes, Bacterial Cloning, Molecular Costs and Cost Analysis DNA, Bacterial/genetics Databases, Factual Genes, Bacterial Genome, Bacterial Haemophilus influenzae/genetics,physiology Molecular Sequence Data Operon RNA, Bacterial/genetics RNA, Ribosomal/genetics Repetitive Sequences, Nucleic Acid Sequence Analysis, DNA/methods Software
Chemicals
Bacterial Proteins DNA, Bacterial RNA, Bacterial RNA, Ribosomal
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Fleischmann R D
Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Adams M D
White O
Clayton R A
Kirkness E F
Kerlavage A R
Bult C J
Tomb J F
Dougherty B A
Merrick J M
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.7542800
Published
1995-07-28
Pages
496-512
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Databases
GENBANK
L75954, L75955, L75956, L75957, L75958, L75959, L75960, L75961, L75962, L75963, L75964, L75965, L75966, L75967, L75968, L75969, L75970, L75971, L75972, L75973, U00069, U00070, U00071, U00072, U00073, U00074, U00075, U00076, U00077, U00078
Corrections
CommentIn
PMID: 7569908
CommentIn
PMID: 7624767
CommentIn
PMID: 8638117
CommentIn
PMID: 8638118
External Links
PubMed source DOI: 10.1126/science.7542800 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2