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PMID: 7565702 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Functional expression of a myo-inositol/H+ symporter from Leishmania donovani.

Molecular and cellular biology ·Vol. 15 ·No. 10 ·1995-10-00 ·Pages 5508-15

Drew ME, Langford CK, Klamo EM, Russell DG, Kavanaugh MP, Landfear SM

Abstract

The vast majority of surface molecules in such kinetoplastid protozoa as members of the genus Leishmania contain inositol and are either glycosyl inositol phospholipids or glycoproteins that are tethered to the external surface of the plasma membrane by glycosylphosphatidylinositol anchors. We have shown that the biosynthetic precursor for these abundant glycolipids, myo-inositol, is translocated across the parasite plasma membrane by a specific transporter that is structurally related to mammalian facilitative glucose transporters. This myo-inositol transporter has been expressed and characterized in Xenopus laevis oocytes. Two-electrode voltage clamp experiments demonstrate that this protein is a sodium-independent electrogenic symporter that appears to utilize a proton gradient to concentrate myo-inositol within the cell. Immunolocalization experiments with a transporter-specific polyclonal antibody reveal the presence of this protein in the parasite plasma membrane.

MeSH Terms
Amino Acid Sequence Animals Biological Transport, Active Carrier Proteins/analysis,biosynthesis,genetics Cell Membrane/chemistry Genes, Protozoan/genetics Hydrogen-Ion Concentration Inositol/metabolism Ion Transport Kinetics Leishmania donovani/metabolism Membrane Proteins/analysis,biosynthesis,genetics Molecular Sequence Data Oocytes Protons Protozoan Proteins/analysis,biosynthesis,genetics Sequence Alignment Symporters Xenopus laevis
Chemicals
Carrier Proteins Membrane Proteins Protons Protozoan Proteins Symporters D1 protein, Leishmania Inositol
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Drew M E
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201, USA.
Langford C K
Klamo E M
Russell D G
Kavanaugh M P
Landfear S M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.10.5508
Published
1995-10-00
Pages
5508-15
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230801
Subset
IM
Grants
NIAID NIH HHS · AI01162 · United States
NIAID NIH HHS · AI25920 · United States
NIGMS NIH HHS · GM48709 · United States
External Links
PubMed source DOI: 10.1128/MCB.15.10.5508 PMC Full Text View journal details
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