Genotypic analysis of multiple loci in somatic cells by whole genome amplification.
To screen multiple loci in small purified samples of diploid and aneuploid cells a PCR-based technique of whole genome amplification was adapted to the study of somatic lesions. DNA samples from different numbers of flow-sorted diploid and aneuploid cells from biopsies were amplified with a degenerate 15mer primer. Aliquots of these reactions were then used in locus-specific reactions using a single round of PCR cycles with individual sets of primers representing polymorphic markers for different regions. As a result, polymorphic markers for different chromosomal regions, including VNTRs and dinucleotide repeats, can be used to perform up to 30 locus-specific PCR assays with a single sample obtained from fewer than 1000 cells.
19 references, click to expand
-
Genetic alterations during colorectal-tumor development.N Engl J Med. 1988 Sep 1;319(9):525-32 PMID: 2841597
-
Clonal ordering of 17p and 5q allelic losses in Barrett dysplasia and adenocarcinoma.Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3221-5 PMID: 8475062
-
Identification of a chromosome 18q gene that is altered in colorectal cancers.Science. 1990 Jan 5;247(4938):49-56 PMID: 2294591
-
Frequent inactivation of the retinoblastoma anti-oncogene is restricted to a subset of human tumor cells.Proc Natl Acad Sci U S A. 1990 Apr;87(7):2775-9 PMID: 2181449
-
Dinucleotide repeat polymorphisms at the D8S85, D8S87, and D8S88 loci.Nucleic Acids Res. 1990 Jul 11;18(13):4038 PMID: 1973840
-
The human mutator gene homolog MSH2 and its association with hereditary nonpolyposis colon cancer.Cell. 1993 Dec 3;75(5):1027-38 PMID: 8252616
-
Mutation in the DNA mismatch repair gene homologue hMLH1 is associated with hereditary non-polyposis colon cancer.Nature. 1994 Mar 17;368(6468):258-61 PMID: 8145827
-
17p allelic losses in diploid cells of patients with Barrett's esophagus who develop aneuploidy.Cancer Res. 1994 May 1;54(9):2292-5 PMID: 8162566
-
Preimplantation single-cell analysis of multiple genetic loci by whole-genome amplification.Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6181-5 PMID: 7517043
-
p53 mutations in human cancers.Science. 1991 Jul 5;253(5015):49-53 PMID: 1905840
-
17p allelic deletions and p53 protein overexpression in Barrett's adenocarcinoma.Cancer Res. 1991 Oct 15;51(20):5482-6 PMID: 1680552
-
A multiple-tubes approach for accurate genotyping of very small DNA samples by using PCR: statistical considerations.Am J Hum Genet. 1992 Feb;50(2):347-59 PMID: 1734715
-
Barrett's esophagus and esophageal adenocarcinoma.Gastroenterol Clin North Am. 1991 Dec;20(4):817-34 PMID: 1787015
-
Clonal expansion of p53 mutant cells is associated with brain tumour progression.Nature. 1992 Feb 27;355(6363):846-7 PMID: 1311419
-
Flow-cytometric and histological progression to malignancy in Barrett's esophagus: prospective endoscopic surveillance of a cohort.Gastroenterology. 1992 Apr;102(4 Pt 1):1212-9 PMID: 1551528
-
Loss of heterozygosity involving the APC and MCC genetic loci occurs in the majority of human esophageal cancers.Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3385-8 PMID: 1565631
-
Whole genome amplification from a single cell: implications for genetic analysis.Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5847-51 PMID: 1631067
-
Loss of heterozygosity involves multiple tumor suppressor genes in human esophageal cancers.Cancer Res. 1992 Dec 1;52(23):6525-30 PMID: 1423299
-
Progression to cancer in Barrett's esophagus is associated with genomic instability.Lab Invest. 1989 Jan;60(1):65-71 PMID: 2911184
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
