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PMID: 7568117 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nuclear magnetic dipole interactions in field-oriented proteins: information for structure determination in solution.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 20 ·1995-09-26 ·Pages 9279-83

Tolman JR, Flanagan JM, Kennedy MA, Prestegard JH

Abstract

The measurement of dipolar contributions to the splitting of 15N resonances of 1H-15N amide pairs in multidimensional high-field NMR spectra of field-oriented cyanometmyoglobin is reported. The splittings appear as small field-dependent perturbations of normal scalar couplings. Assignment of more than 90 resonances to specific sequential sites in the protein allows correlation of the dipolar contributions with predictions based on the known susceptibility and known structure of the protein. Implications as an additional source of information for protein structure determination in solution are discussed.

MeSH Terms

Amino Acid Sequence Cloning, Molecular Escherichia coli Heme/analysis Hydrogen Magnetic Resonance Spectroscopy/methods Metmyoglobin/analogs & derivatives,chemistry Models, Molecular Molecular Sequence Data Myoglobin/biosynthesis,chemistry,isolation & purification Nitrogen Isotopes Protein Structure, Secondary Proteins/chemistry Recombinant Proteins/biosynthesis,chemistry,isolation & purification Software Solutions

Chemicals

Myoglobin Nitrogen Isotopes Proteins Recombinant Proteins Solutions cyanometmyoglobin Metmyoglobin Heme Hydrogen

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Tolman J R

Department of Chemistry, Yale University, New Haven, CT 06520-8107, USA.

Flanagan J M

Kennedy M A

Prestegard J H

References (10)

10 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.92.20.9279

Published

1995-09-26

Pages

9279-83

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC40968

Subset

Grants

NIGMS NIH HHS · GM33225 · United States

External Links

PubMed source DOI: 10.1073/pnas.92.20.9279 PMC Full Text View journal details

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Nuclear magnetic dipole interactions in field-oriented proteins: information for structure determination in solution.

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