Abstract
In this report we structurally and functionally define a binding domain that is involved in protein association and that we have designated EH (for Eps15 homology domain). This domain was identified in the tyrosine kinase substrate Eps15 on the basis of regional conservation with several heterogeneous proteins of yeast and nematode. The EH domain spans about 70 amino acids and shows approximately 60% overall amino acid conservation. We demonstrated the ability of the EH domain to specifically bind cytosolic proteins in normal and malignant cells of mesenchymal, epithelial, and hematopoietic origin. These observations prompted our search for additional EH-containing proteins in mammalian cells. Using an EH domain-specific probe derived from the eps15 cDNA, we cloned and characterized a cDNA encoding an EH-containing protein with overall similarity to Eps15; we designated this protein Eps15r (for Eps15-related). Structural comparison of Eps15 and Eps15r defines a family of signal transducers possessing extensive networking abilities including EH-mediated binding and association with Src homology 3-containing proteins.
MeSH Terms
Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Animals
Biological Evolution
Calcium-Binding Proteins/genetics,metabolism
Cells, Cultured
Conserved Sequence
Intracellular Signaling Peptides and Proteins
Mice
Molecular Sequence Data
Peptide Fragments/genetics,metabolism
Phosphoproteins/genetics,metabolism
Protein Binding
Receptor Protein-Tyrosine Kinases/metabolism
Recombinant Proteins/metabolism
Sequence Homology, Amino Acid
Species Specificity
Chemicals
Adaptor Proteins, Signal Transducing
Calcium-Binding Proteins
Eps15 protein, mouse
Intracellular Signaling Peptides and Proteins
Peptide Fragments
Phosphoproteins
Recombinant Proteins
Receptor Protein-Tyrosine Kinases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wong W T
Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Schumacher C
Salcini A E
Romano A
Castagnino P
Pelicci P G
Di Fiore P P
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