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PMID: 7568191 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

TRPC1, a human homolog of a Drosophila store-operated channel.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 21 ·1995-10-10 ·Pages 9652-6

Wes PD, Chevesich J, Jeromin A, Rosenberg C, Stetten G, Montell C

Abstract

In many vertebrate and invertebrate cells, inositol 1,4,5-trisphospate production induces a biphasic Ca2+ signal. Mobilization of Ca2+ from internal stores drives the initial burst. The second phase, referred to as store-operated Ca2+ entry (formerly capacitative Ca2+ entry), occurs when depletion of intracellular Ca2+ pools activates a non-voltage-sensitive plasma membrane Ca2+ conductance. Despite the prevalence of store-operated Ca2+ entry, no vertebrate channel responsible for store-operated Ca2+ entry has been reported. trp (transient receptor potential), a Drosophila gene required in phototransduction, encodes the only known candidate for such a channel throughout phylogeny. In this report, we describe the molecular characterization of a human homolog of trp, TRPC1. TRPC1 (transient receptor potential channel-related protein 1) was 40% identical to Drosophila TRP over most of the protein and lacked the charged residues in the S4 transmembrane region proposed to be required for the voltage sensor in many voltage-gated ion channels. TRPC1 was expressed at the highest levels in the fetal brain and in the adult heart, brain, testis, and ovaries. Evidence is also presented that TRPC1 represents the archetype of a family of related human proteins.

MeSH Terms
Adult Amino Acid Sequence Animals Biological Evolution Calcium Channels/genetics Chromosomes, Human, Pair 3 Conserved Sequence DNA, Complementary/genetics Drosophila Drosophila Proteins Embryo, Mammalian Embryo, Nonmammalian Genomic Library Humans In Situ Hybridization, Fluorescence Insect Hormones/genetics Insect Proteins Ion Channels/genetics Membrane Proteins/genetics Molecular Sequence Data Multigene Family Protein Conformation Rats Sequence Analysis, DNA Sequence Homology, Amino Acid TRPC Cation Channels Tissue Distribution Transient Receptor Potential Channels
Chemicals
Calcium Channels DNA, Complementary Drosophila Proteins Insect Hormones Insect Proteins Ion Channels Membrane Proteins TRPC Cation Channels Transient Receptor Potential Channels transient receptor potential cation channel, subfamily C, member 1 trp protein, Drosophila
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Wes P D
Department of Biological Chemistry, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.
Chevesich J
Jeromin A
Rosenberg C
Stetten G
Montell C
References (43)
43 references, click to expand
  1. Modular organization of actin crosslinking proteins.
    Trends Biochem Sci. 1991 Mar;16(3):87-92 PMID: 2058002
  2. Activation of Ca2+ entry into acinar cells by a non-phosphorylatable inositol trisphosphate.
    Nature. 1991 Jul 11;352(6331):162-5 PMID: 1648669
  3. Abnormal electroretinogram from a Drosophila mutant.
    Nature. 1969 Oct 18;224(5216):285-7 PMID: 5344615
  4. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  5. Rescue of the Drosophila phototransduction mutation trp by germline transformation.
    Science. 1985 Nov 29;230(4729):1040-3 PMID: 3933112
  6. A model for receptor-regulated calcium entry.
    Cell Calcium. 1986 Feb;7(1):1-12 PMID: 2420465
  7. Fluorescence detection in automated DNA sequence analysis.
    Nature. 1986 Jun 12-18;321(6071):674-9 PMID: 3713851
  8. Proper function of the Drosophila trp gene product during pupal development is important for normal visual transduction in the adult.
    Neuron. 1989 Jul;3(1):81-94 PMID: 2482778
  9. Molecular characterization of the Drosophila trp locus: a putative integral membrane protein required for phototransduction.
    Neuron. 1989 Apr;2(4):1313-23 PMID: 2516726
  10. 'Quantal' Ca2+ release and the control of Ca2+ entry by inositol phosphates--a possible mechanism.
    FEBS Lett. 1990 Apr 9;263(1):5-9 PMID: 2185036
  11. Calcium oscillations.
    J Biol Chem. 1990 Jun 15;265(17):9583-6 PMID: 2161835
  12. Predicting coiled coils from protein sequences.
    Science. 1991 May 24;252(5009):1162-4 PMID: 2031185
  13. Capacitative calcium entry revisited.
    Cell Calcium. 1990 Nov-Dec;11(10):611-24 PMID: 1965707
  14. Depletion of intracellular calcium stores activates a calcium current in mast cells.
    Nature. 1992 Jan 23;355(6358):353-6 PMID: 1309940
  15. Analysis of reciprocal translocations by chromosome painting: applications and limitations of the technique.
    Am J Hum Genet. 1992 Apr;50(4):700-5 PMID: 1550115
  16. Identification of a Drosophila gene encoding a calmodulin-binding protein with homology to the trp phototransduction gene.
    Neuron. 1992 Apr;8(4):631-42 PMID: 1314616
  17. The trp gene is essential for a light-activated Ca2+ channel in Drosophila photoreceptors.
    Neuron. 1992 Apr;8(4):643-51 PMID: 1314617
  18. Ankyrins. Adaptors between diverse plasma membrane proteins and the cytoplasm.
    J Biol Chem. 1992 May 5;267(13):8703-6 PMID: 1533619
  19. Tracing the roots of ion channels.
    Cell. 1992 May 29;69(5):715-8 PMID: 1375539
  20. Rapid and reliable fluorescent cycle sequencing of double-stranded templates.
    DNA Seq. 1992;2(5):289-96 PMID: 1633326
  21. Inositol trisphosphate and calcium signalling.
    Nature. 1993 Jan 28;361(6410):315-25 PMID: 8381210
  22. Detergent solubility of the inositol trisphosphate receptor in rat brain membranes. Evidence for association of the receptor with ankyrin.
    J Biol Chem. 1993 Mar 25;268(9):6477-86 PMID: 8384211
  23. The involvement of ankyrin in the regulation of inositol 1,4,5-trisphosphate receptor-mediated internal Ca2+ release from Ca2+ storage vesicles in mouse T-lymphoma cells.
    J Biol Chem. 1993 Apr 5;268(10):7290-7 PMID: 8385102
  24. Calcium influx and its control by calcium release.
    Curr Opin Neurobiol. 1993 Jun;3(3):368-74 PMID: 8396477
  25. The signal for capacitative calcium entry.
    Cell. 1993 Oct 22;75(2):199-201 PMID: 8402906
  26. Excitement about calcium signaling in inexcitable cells.
    Science. 1993 Oct 29;262(5134):676-8 PMID: 8235587
  27. Novel Ca2+ channels underlying transduction in Drosophila photoreceptors: implications for phosphoinositide-mediated Ca2+ mobilization.
    Trends Neurosci. 1993 Sep;16(9):371-6 PMID: 7694408
  28. 2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans.
    Nature. 1994 Mar 3;368(6466):32-8 PMID: 7906398
  29. Hundreds of ankyrin-like repeats in functionally diverse proteins: mobile modules that cross phyla horizontally?
    Proteins. 1993 Dec;17(4):363-74 PMID: 8108379
  30. Receptor-activated Ca2+ influx: how many mechanisms for how many channels?
    Trends Pharmacol Sci. 1994 Mar;15(3):77-83 PMID: 8184490
  31. Appearance of a novel Ca2+ influx pathway in Sf9 insect cells following expression of the transient receptor potential-like (trpl) protein of Drosophila.
    Biochem Biophys Res Commun. 1994 Jun 15;201(2):1050-6 PMID: 7516156
  32. The light response of Drosophila photoreceptors is accompanied by an increase in cellular calcium: effects of specific mutations.
    Neuron. 1994 Jun;12(6):1257-67 PMID: 8011336
  33. Calcium channels activated by depletion of internal calcium stores in A431 cells.
    Biophys J. 1994 Jul;67(1):177-82 PMID: 7918985
  34. Activation of recombinant trp by thapsigargin in Sf9 insect cells.
    Am J Physiol. 1994 Nov;267(5 Pt 1):C1501-5 PMID: 7977711
  35. Receptor-mediated activation of recombinant Trpl expressed in Sf9 insect cells.
    Biochem J. 1995 Jan 15;305 ( Pt 2):605-11 PMID: 7832780
  36. Calcium signaling.
    Cell. 1995 Jan 27;80(2):259-68 PMID: 7834745
  37. The Drosophila cation channel trpl expressed in insect Sf9 cells is stimulated by agonists of G-protein-coupled receptors.
    FEBS Lett. 1995 Jan 30;358(3):297-300 PMID: 7531160
  38. Arc, a growth factor and activity-regulated gene, encodes a novel cytoskeleton-associated protein that is enriched in neuronal dendrites.
    Neuron. 1995 Feb;14(2):433-45 PMID: 7857651
  39. Genetic dissection of light-induced Ca2+ influx into Drosophila photoreceptors.
    J Gen Physiol. 1994 Dec;104(6):1057-77 PMID: 7699363
  40. Pore loops: an emerging theme in ion channel structure.
    Neuron. 1995 May;14(5):889-92 PMID: 7538310
  41. TRP, a protein essential for inositide-mediated Ca2+ influx is localized adjacent to the calcium stores in Drosophila photoreceptors.
    J Neurosci. 1995 May;15(5 Pt 2):3747-60 PMID: 7751943
  42. Intracellular calcium. Replenishing the stores.
    Nature. 1995 Jun 22;375(6533):634-5 PMID: 7791893
  43. Ion channels and calcium signaling in mast cells.
    Ann N Y Acad Sci. 1993 Dec 20;707:198-209 PMID: 9137553
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.21.9652
Published
1995-10-10
Pages
9652-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40860
Subset
IM
Grants
NEI NIH HHS · EY10852 · United States
NICHD NIH HHS · HD24605 · United States
Databases
GENBANK
X89066, X89067, X89068
External Links
PubMed source DOI: 10.1073/pnas.92.21.9652 PMC Full Text View journal details
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